WFRF:(Båverud Viveca)
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LIBRIS Formathandbok (Information om MARC21)
| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03536naa a2200421 4500 | |
| 001 | oai:DiVA.org:uu-159516 | |
| 003 | SwePub | |
| 008 | 111003s2011 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1595162 URI |
| 024 | 7 | a https://doi.org/10.1099/jmm.0.031179-02 DOI |
| 040 | a (SwePub)uu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Hedeland, Mikaelu Uppsala universitet,Avdelningen för analytisk farmaceutisk kemi4 aut0 (Swepub:uu)mikahede |
| 245 | 1 0 | a Confirmation of botulism in birds and cattle by the mouse bioassay and Endopep-MS |
| 264 | 1 | b Microbiology Society,c 2011 |
| 338 | a print2 rdacarrier | |
| 520 | a There have been several outbreaks of botulism among poultry and wild birds in Sweden in recent years. The National Veterinary Institute of Sweden (SVA) has identified botulinum neurotoxin (BoNT)/C1 or the mosaic BoNT/C1D using the mouse bioassay. This is believed to be the first report on the application of the Endopep mass spectrometry (Endopep-MS) method to selected clinical animal (serum and liver) samples and a feed sample that had previously given positive test results with the mouse bioassay. In the mouse bioassay eight of the eleven samples were found to be neutralized by both BoNT/C1 and /D antitoxins; the other three were neutralized only by BoNT/C1 antitoxin, but the mice showed a prolonged survival time when the samples had been treated with /D antitoxin. The Endopep-MS analysis, on the other hand, demonstrated only BoNT/C1 activity for all eleven samples. This suggests that at least eight of the samples were of the chimeric toxin type BoNT/C1D, where the enzymically active site is identical to that of BoNT/C1, while other parts of the protein contain sequences of BoNT/D. This is the first step of a cross-validation between the established mouse bioassay and the Endopep-MS of serotypes BoNT/C1 and /C1D. Endopep-MS is concluded to have potential as an attractive alternative to the mouse bioassay. | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Läkemedelskemi0 (SwePub)301032 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Medicinal Chemistry0 (SwePub)301032 hsv//eng |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Farmaceutiska vetenskaper0 (SwePub)301012 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Pharmaceutical Sciences0 (SwePub)301012 hsv//eng |
| 653 | a Pharmaceutical chemistry | |
| 653 | a Farmaceutisk kemi | |
| 653 | a Analytical Pharmaceutical Chemistry | |
| 653 | a Analytisk farmaceutisk kemi | |
| 700 | 1 | a Moura, Hercules4 aut |
| 700 | 1 | a Båverud, Viveca4 aut |
| 700 | 1 | a Woolfitt, Adrian R4 aut |
| 700 | 1 | a Bondesson, Ulfu Uppsala universitet,Avdelningen för analytisk farmaceutisk kemi4 aut0 (Swepub:uu)ulfbonde |
| 700 | 1 | a Barr, John R.4 aut |
| 710 | 2 | a Uppsala universitetb Avdelningen för analytisk farmaceutisk kemi4 org |
| 773 | 0 | t Journal of Medical Microbiologyd : Microbiology Societyg 60:9, s. 1299-1305q 60:9<1299-1305x 0022-2615x 1473-5644 |
| 856 | 4 | u http://jmm.microbiologyresearch.org/deliver/fulltext/jmm/60/9/1299_jmm031179.pdf?itemId=/content/journal/jmm/10.1099/jmm.0.031179-0&mimeType=pdf&isFastTrackArticle= |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-159516 |
| 856 | 4 8 | u https://doi.org/10.1099/jmm.0.031179-0 |
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