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Salmonella persiste...
Salmonella persisters promote the spread of antibiotic resistance plasmids in the gut
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- Bakkeren, Erik (författare)
- Swiss Fed Inst Technol, Dept Biol, Inst Microbiol, Zurich, Switzerland
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- Huisman, Jana S. (författare)
- Swiss Fed Inst Technol, Inst Integrat Biol, Dept Environm Syst Sci, Zurich, Switzerland;Swiss Inst Bioinformat, Lausanne, Switzerland
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- Fattinger, Stefan A. (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Swiss Fed Inst Technol, Dept Biol, Inst Microbiol, Zurich, Switzerland;Uppsala Univ, Sci Life Lab, Dept Med Biochem & Microbiol, Uppsala, Sweden
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- Hausmann, Annika (författare)
- Swiss Fed Inst Technol, Dept Biol, Inst Microbiol, Zurich, Switzerland
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- Furter, Markus (författare)
- Swiss Fed Inst Technol, Dept Biol, Inst Microbiol, Zurich, Switzerland
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- Egli, Adrian (författare)
- Univ Hosp Basel, Div Clin Microbiol, Basel, Switzerland;Univ Basel, Dept Biomed, Appl Microbiol Res, Basel, Switzerland
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- Slack, Emma (författare)
- Swiss Fed Inst Technol, Dept Biol, Inst Microbiol, Zurich, Switzerland;Swiss Fed Inst Technol, Inst Food Nutr & Hlth, Dept Hlth Sci & Technol, Zurich, Switzerland
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- Sellin, Mikael E. (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Bonhoeffer, Sebastian (författare)
- Swiss Fed Inst Technol, Inst Integrat Biol, Dept Environm Syst Sci, Zurich, Switzerland
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- Regoes, Roland R. (författare)
- Swiss Fed Inst Technol, Inst Integrat Biol, Dept Environm Syst Sci, Zurich, Switzerland
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- Diard, Mederic (författare)
- Swiss Fed Inst Technol, Dept Biol, Inst Microbiol, Zurich, Switzerland;Univ Basel, Biozentrum, Basel, Switzerland
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- Hardt, Wolf-Dietrich (författare)
- Swiss Fed Inst Technol, Dept Biol, Inst Microbiol, Zurich, Switzerland
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(creator_code:org_t)
- 2019-09-04
- 2019
- Engelska.
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Ingår i: Nature. - : NATURE PUBLISHING GROUP. - 0028-0836 .- 1476-4687. ; 573:7773, s. 276-280
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- The emergence of antibiotic-resistant bacteria through mutations or the acquisition of genetic material such as resistance plasmids represents a major public health issue(1,2). Persisters are subpopulations of bacteria that survive antibiotics by reversibly adapting their physiology(3-10), and can promote the emergence of antibiotic-resistant mutants(11). We investigated whether persisters can also promote the spread of resistance plasmids. In contrast to mutations, the transfer of resistance plasmids requires the co-occurrence of both a donor and a recipient bacterial strain. For our experiments, we chose the facultative intracellular entero-pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium) and Escherichia coli, a common member of the microbiota(12). S. Typhimurium forms persisters that survive antibiotic therapy in several host tissues. Here we show that tissue-associated S. Typhimurium persisters represent long-lived reservoirs of plasmid donors or recipients. The formation of reservoirs of S. Typhimurium persisters requires Salmonella pathogenicity island (SPI)-1 and/or SPI-2 in gut-associated tissues, or SPI-2 at systemic sites. The re-seeding of these persister bacteria into the gut lumen enables the co-occurrence of donors with gut-resident recipients, and thereby favours plasmid transfer between various strains of Enterobacteriaceae. We observe up to 99% transconjugants within two to three days of re-seeding. Mathematical modelling shows that rare re-seeding events may suffice for a high frequency of conjugation. Vaccination reduces the formation of reservoirs of persisters after oral infection with S. Typhimurium, as well as subsequent plasmid transfer. We conclude that-even without selection for plasmid-encoded resistance genes-small reservoirs of pathogen persisters can foster the spread of promiscuous resistance plasmids in the gut.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
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Bakkeren, Erik
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Huisman, Jana S.
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Fattinger, Stefa ...
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Hausmann, Annika
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Furter, Markus
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Egli, Adrian
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Slack, Emma
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Sellin, Mikael E ...
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Bonhoeffer, Seba ...
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Regoes, Roland R ...
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Diard, Mederic
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Hardt, Wolf-Diet ...
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