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Effect of extraction routes on protein content, solubility and molecular weight distribution of Crambe abyssinica protein concentrates and thermally processed films thereof

Newson, William (author)
Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtförädling,Department of Plant Breeding
Prieto-Linde, Maria Luisa (author)
Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtförädling,Department of Plant Breeding
Kuktaite, Ramune (author)
Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtförädling,Department of Plant Breeding
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Hedenqvist, Mikael S. (author)
KTH,Fiber- och polymerteknologi,KTH Royal Institute of Technology, Sweden
Gällstedt, Mikael (author)
RISE,Bioekonomi,Innventia
Johansson, Eva (author)
Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtförädling,Department of Plant Breeding
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 (creator_code:org_t)
 
Elsevier BV, 2017
2017
English.
In: Industrial crops and products (Print). - : Elsevier BV. - 0926-6690 .- 1872-633X. ; 97, s. 591-598
Related links:
https://urn.kb.se/re...
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https://doi.org/10.1...
https://urn.kb.se/re...
https://res.slu.se/i...
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  • Journal article (peer-reviewed)
Abstract Subject headings
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  • To understand if and how extraction conditions influence properties of molded protein films, Crambe abyssinica protein concentrates were produced from deoiled seed meal under various extraction conditions. Properties of the resulting hot compression molded films were evaluated through the molecular weight distribution, protein polymerization behavior and tensile tests. Precipitated protein concentrates demonstrated higher protein content and a pronounced shift to higher molecular weight distributions and reduced solubility on heating, indicating increased protein polymerization compared to those from lyophilized supernatants. Thermally processed films from isoelectrically precipitated protein concentrates show a high resistance to extraction with a combination of reducing agent and denaturant, indicating the presence of non-disulfide covalent cross linking. Also, tensile strength was higher in concentrates from precipitated proteins compared to those from supernatants. The protein concentrates resulting in thermally processed films with a high protein content, the highest levels of protein-protein interaction and high tensile strength were based on alkaline extraction and isoelectric precipitation. Therefore, a combination of alkali extraction and isoelectric precipitation is recommended to produce protein concentrates for molded film production.

Subject headings

TEKNIK OCH TEKNOLOGIER  -- Materialteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY  -- Materials Engineering (hsv//eng)
TEKNIK OCH TEKNOLOGIER  -- Kemiteknik -- Polymerteknologi (hsv//swe)
ENGINEERING AND TECHNOLOGY  -- Chemical Engineering -- Polymer Technologies (hsv//eng)
LANTBRUKSVETENSKAPER  -- Lantbruksvetenskap, skogsbruk och fiske -- Jordbruksvetenskap (hsv//swe)
AGRICULTURAL SCIENCES  -- Agriculture, Forestry and Fisheries -- Agricultural Science (hsv//eng)

Keyword

Compression molding
Extraction
Molecular weight
Molecular weight distribution
Polymerization
Solubility
Tensile strength
Tensile testing

Publication and Content Type

ref (subject category)
art (subject category)

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