Microfluidic Diffusion Analysis of the Sizes and Interactions of Proteins under Native Solution Conditions.

Arosio, Paolo (author)

Müller, Thomas (author)

Rajah, Luke (author)

Yates, Emma V (author)

Aprile, Francesco A (author)

Zhang, Yingbo (author)

Cohen, Samuel I A (author)

White, Duncan A (author)

Herling, Therese W (author)

De Genst, Erwin J (author)

Linse, Sara (author): Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH

Vendruscolo, Michele (author)

Dobson, Christopher M (author)

Knowles, Tuomas P J (author)

(creator_code:org_t)

2015-12-23
2016
English 9 s.
In: ACS Nano. - : American Chemical Society (ACS). - 1936-086X .- 1936-0851. ; 10:1, s. 333-341

Related links:: http://dx.doi.org/10...; show more...; https://lup.lub.lu.s...; https://doi.org/10.1...; show less...

Abstract Subject headings

Characterizing the sizes and interactions of macromolecules under native conditions is a challenging problem in many areas of molecular sciences, which fundamentally arises from the polydisperse nature of biomolecular mixtures. Here, we describe a microfluidic platform for diffusional sizing based on monitoring micron-scale mass transport simultaneously in space and time. We show that the global analysis of such combined space-time data enables the hydrodynamic radii of individual species within mixtures to be determined directly by deconvoluting average signals into the contributions from the individual species. We demonstrate that the ability to perform rapid noninvasive sizing allows this method to be used to characterize interactions between biomolecules under native conditions. We illustrate the potential of the technique by implementing a single-step quantitative immunoassay that operates on a time scale of seconds and detects specific interactions between biomolecules within complex mixtures.

By the author/editor: Arosio, Paolo; Müller, Thomas; Rajah, Luke; Yates, Emma V; Aprile, Francesc ...; Zhang, Yingbo; show more...; Cohen, Samuel I ...; White, Duncan A; Herling, Therese ...; De Genst, Erwin ...; Linse, Sara; Vendruscolo, Mic ...; Dobson, Christop ...; Knowles, Tuomas ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Analytical Chemi ...

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