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Toward Rapid Detect...
Toward Rapid Detection of Viable Bacteria in Whole Blood for Early Sepsis Diagnostics and Susceptibility Testing
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- Iyengar, Sharath Narayana (author)
- KTH,Science for Life Laboratory, SciLifeLab,Nanobioteknologi,Karolinska Inst, AIMES Ctr Adv Integrated Med & Engn Sci, S-17165 Stockholm, Sweden.;KTH Royal Inst Technol, S-17165 Stockholm, Sweden.
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- Dietvorst, Jiri (author)
- Univ Autonoma Barcelona, Inst Microelect Barcelona IMB CNM, CSIC, Barcelona 08193, Spain.
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- Ferrer-Vilanova, Amparo (author)
- Univ Autonoma Barcelona, Inst Microelect Barcelona IMB CNM, CSIC, Barcelona 08193, Spain.
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- Guirado, Gonzalo (author)
- Univ Autonoma Barcelona, Dept Quim, Barcelona 08193, Spain.
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- Munoz-Berbel, Xavier (author)
- Univ Autonoma Barcelona, Inst Microelect Barcelona IMB CNM, CSIC, Barcelona 08193, Spain.
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- Russom, Aman, Prof. 1976- (author)
- Karolinska Institutet,KTH,Science for Life Laboratory, SciLifeLab,Nanobioteknologi,Div Nanobiotechnol, Dept Prot Sci, Sci Life Lab, S-17165 Stockholm, Sweden.;Karolinska Inst, AIMES Ctr Adv Integrated Med & Engn Sci, S-17165 Stockholm, Sweden.;KTH Royal Inst Technol, S-17165 Stockholm, Sweden.
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(creator_code:org_t)
- 2021-08-19
- 2021
- English.
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In: ACS Sensors. - : American Chemical Society (ACS). - 2379-3694. ; 6:9, s. 3357-3366
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Abstract
Subject headings
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- Sepsis is a serious bloodstream infection where the immunity of the host body is compromised, leading to organ failure and death of the patient. In early sepsis, the concentration of bacteria is very low and the time of diagnosis is very critical since mortality increases exponentially with every hour after infection. Common culture-based methods fail in fast bacteria determination, while recent rapid diagnostic methods are expensive and prone to false positives. In this work, we present a sepsis kit for fast detection of bacteria in whole blood, here achieved by combining selective cell lysis and a sensitive colorimetric approach detecting as low as 10(3) CFU/mL bacteria in less than 5 h. Homemade selective cell lysis buffer (combination of saponin and sodium cholate) allows fast processing of whole blood in 5 min while maintaining bacteria alive (100% viability). After filtration, retained bacteria on filter paper are incubated under constant illumination with the electrochromic precursors, i.e., ferricyanide and ferric ammonium citrate. Viable bacteria metabolically reduce iron(III) complexes, initiating a photocatalytic cascade toward Prussian blue formation. As a proof of concept, we combine this method with antibiotic susceptibility testing to determine the minimum inhibitory concentration (MIC) using two antibiotics (ampicillin and gentamicin). Although this kit is used to demonstrate its applicability to sepsis, this approach is expected to impact other key sectors such as hygiene evaluation, microbial contaminated food/beverage, or UTI, among others.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- sepsis
- bacteria
- E. coli
- selective cell lysis
- Prussian blue
- colorimetric
- blood
Publication and Content Type
- ref (subject category)
- art (subject category)
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