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Engraftment of engi...
Engraftment of engineered ES cell-derived cardiomyocytes but not BM cells restores contractile function to the infarcted myocardium
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- Kolossov, Eugen (författare)
- Axiogenesis AG, 50931 Cologne, Germany
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- Bostani, Toktam (författare)
- Institute of Physiology I, University of Bonn, 53105 Bonn, Germany
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- Roell, Wilhelm (författare)
- Department of Cardiac Surgery, University of Bonn, 53105 Bonn, Germany
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- Breitbach, Martin (författare)
- Institute of Physiology I, University of Bonn, 53105 Bonn, Germany
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- Pillekamp, Frank (författare)
- Institute of Neurophysiology, University of Cologne, 50931 Cologne, Germany
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- Nygren, Jens Martin, 1976- (författare)
- Lund University,Lunds universitet,Medicinska fakulteten,Faculty of Medicine,Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, 221 00 Lund, Sweden
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- Sasse, Philipp (författare)
- Institute of Physiology I, University of Bonn, 53105 Bonn, Germany
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- Rubenchik, Olga (författare)
- Institute of Neurophysiology, University of Cologne, 50931 Cologne, Germany
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- Fries, Jochen W U (författare)
- Department Pathology, University of Cologne, 50931 Cologne, Germany
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- Wenzel, Daniela (författare)
- Institute of Physiology I, University of Bonn, 53105 Bonn, Germany
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- Geisen, Caroline (författare)
- Institute of Physiology I, University of Bonn, 53105 Bonn, Germany
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- Xia, Ying (författare)
- Institute of Neurophysiology, University of Cologne, 50931 Cologne, Germany
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- Lu, Zhongju (författare)
- Institute of Neurophysiology, University of Cologne, 50931 Cologne, Germany
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- Duan, Yaqi (författare)
- Institute of Neurophysiology, University of Cologne, 50931 Cologne, Germany
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- Kettenhofen, Ralf (författare)
- Axiogenesis AG, 50931 Cologne, Germany
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- Jovinge, Stefan (författare)
- Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine,Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, 221 00 Lund, Sweden
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- Bloch, Wilhelm (författare)
- German Sports University, 50927 Cologne, Germany
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- Bohlen, Heribert (författare)
- Axiogenesis AG, 50931 Cologne, Germany
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- Welz, Armin (författare)
- Department of Cardiac Surgery, University of Bonn, 53105 Bonn, Germany
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- Hescheler, Jürgen (författare)
- Institute of Neurophysiology, University of Cologne, 50931 Cologne, Germany
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- Jacobsen, Sten Eirik W (författare)
- Lund University,Lunds universitet,Stamcellscentrum (SCC),Avdelningen för stamcellsforskning,Institutionen för laboratoriemedicin,Medicinska fakulteten,Stem Cell Center,Division of stem cell research,Department of Laboratory Medicine,Faculty of Medicine,Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, 221 00 Lund, Sweden
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- Fleischmann, Bernd K (författare)
- Institute of Physiology I, University of Bonn, 53105 Bonn, Germany
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(creator_code:org_t)
- 2006-09-05
- 2006
- Engelska.
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Ingår i: Journal of Experimental Medicine. - New York, USA : Rockefeller University Press. - 0022-1007 .- 1540-9538. ; 203:10, s. 2315-2327
- Relaterad länk:
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http://jem.rupress.o...
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http://dx.doi.org/10...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- Cellular cardiomyoplasty is an attractive option for the treatment of severe heart failure. It is, however, still unclear and controversial which is the most promising cell source. Therefore, we investigated and examined the fate and functional impact of bone marrow (BM) cells and embryonic stem cell (ES cell)-derived cardiomyocytes after transplantation into the infarcted mouse heart. This proved particularly challenging for the ES cells, as their enrichment into cardiomyocytes and their long-term engraftment and tumorigenicity are still poorly understood. We generated transgenic ES cells expressing puromycin resistance and enhanced green fluorescent protein cassettes under control of a cardiac-specific promoter. Puromycin selection resulted in a highly purified (>99%) cardiomyocyte population, and the yield of cardiomyocytes increased 6-10-fold because of induction of proliferation on purification. Long-term engraftment (4-5 months) was observed when co-transplanting selected ES cell-derived cardiomyocytes and fibroblasts into the injured heart of syngeneic mice, and no teratoma formation was found (n = 60). Although transplantation of ES cell-derived cardiomyocytes improved heart function, BM cells had no positive effects. Furthermore, no contribution of BM cells to cardiac, endothelial, or smooth muscle neogenesis was detected. Hence, our results demonstrate that ES-based cell therapy is a promising approach for the treatment of impaired myocardial function and provides better results than BM-derived cells.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Animals
- Bone Marrow Transplantation
- DNA Primers
- Electrophysiology
- Embryonic Stem Cells
- Green Fluorescent Proteins
- Immunohistochemistry
- Mice
- Myocardial Contraction
- Myocardial Infarction
- Myocytes
- Cardiac
- Puromycin
- Reverse Transcriptase Polymerase Chain Reaction
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
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Kolossov, Eugen
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Bostani, Toktam
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Roell, Wilhelm
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Breitbach, Marti ...
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Pillekamp, Frank
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Nygren, Jens Mar ...
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visa fler...
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Sasse, Philipp
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Rubenchik, Olga
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Fries, Jochen W ...
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Wenzel, Daniela
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Geisen, Caroline
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Xia, Ying
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Lu, Zhongju
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Duan, Yaqi
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Kettenhofen, Ral ...
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Jovinge, Stefan
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Bloch, Wilhelm
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Bohlen, Heribert
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Welz, Armin
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Hescheler, Jürge ...
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Jacobsen, Sten E ...
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Fleischmann, Ber ...
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- MEDICIN OCH HÄLSOVETENSKAP
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och Cell och molekyl ...
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- Av lärosätet
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Högskolan i Halmstad
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Lunds universitet