Sökning: WFRF:(Jensen Jørgen Skov) >
Sequence-based typi...
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Hjorth, SVStatens Serum Institut, DK-2300 Copenhagen
(författare)
Sequence-based typing of Mycoplasma genitalium reveals sexual transmission
- Artikel/kapitelEngelska2006
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:liu-43014
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-43014URI
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https://doi.org/10.1128/JCM.00003-06DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:1932918URI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Mycoplasma genitalium causes male nonchlamydial, nongonococcal urethritis and is associated with cervicitis and pelvic inflammatory disease in women. Epidemiological studies indicate that M. genitalium is sexually transmitted, and the aim of the present study was to further substantiate this by means of a DNA typing system. A typing assay based on a diagnostic mgpB gene PCR was developed, evaluated, and applied directly to urogenital specimens. The assay had a low limit of detection and hence a high typeability. Sequences of isolates from 52 unrelated patients were divided into 29 different sequence types, giving a discriminatory index of 0.95. Two to six M. genitalium-positive specimens were collected from each of 44 patients over a median interval of 56 days (range, 11 to 1,395). Forty had the same sequence type in consecutive specimens. Specimens collected from two men were repeatedly positive at intervals of 472 and 1,395 days, respectively, but the sequence types had changed. A new strain was introduced in one sexual dyad, and the sequence types changed subsequently. Seventy-nine M. genitalium-positive specimens from 19 couples were investigated, and all partners initially had concordant sequence types, but one couple had discordant types at one time point before a newly introduced strain took over. The present typing system is simple and reproducible and has an excellent discriminatory capacity which might prove useful in studies of sexual networks and for evaluation of treatment failures. In the laboratory, this system may document the uniqueness of newly isolated M. genitalium strains.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Björnelius, EvaKarolinska Institutet
(författare)
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Lidbrink, PeterKarolinska Institutet
(författare)
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Falk, Lars,1954-Örebro University Hospital(Swepub:liu)larfa68
(författare)
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Dohn, BStatens Serum Institut, DK-2300 Copenhagen
(författare)
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Berthelsen, LStatens Serum Institut, DK-2300 Copenhagen
(författare)
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Ma, LLouisiana State University Health Sciences Center
(författare)
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Martin, DHLouisiana State University Health Sciences Center
(författare)
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Jensen, Jörgen SkovStatens Serum Institut, DK-2300 Copenhagen
(författare)
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Karolinska InstitutetStatens Serum Institut, DK-2300 Copenhagen
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Clinical Microbiology44:6, s. 2078-20830095-11371098-660X
Internetlänk
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Till lärosätets databas
- Av författaren/redakt...
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Hjorth, SV
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Björnelius, Eva
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Lidbrink, Peter
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Falk, Lars, 1954 ...
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Dohn, B
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Berthelsen, L
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visa fler...
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Ma, L
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Martin, DH
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Jensen, Jörgen S ...
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visa färre...
- Artiklar i publikationen
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Journal of Clini ...
- Av lärosätet
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Linköpings universitet
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Karolinska Institutet