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Protein Modificatio...
Protein Modifications by Low Molecular Weight Chemicals Inducing Airways Diseases
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- Jeppsson, Marina (författare)
- Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine
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(creator_code:org_t)
- ISBN 9789186443238
- 2010
- Engelska 138 s.
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Serie: Lund University Faculty of Medicine Doctoral Dissertation Series, 1652-8220
- Relaterad länk:
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https://lup.lub.lu.s...
Abstract
Ämnesord
Stäng
- Airways diseases are one of the most common health effects in the western world today. It has been shown that low molecular weight chemicals (LMWC) can cause airways diseases. The mechanisms for these diseases are not well known in most cases. Also, methods for biological monitoring of exposure are not available for many of these LMWC. This limits the possibility for diagnostics and prevention. The binding of LMWC to endogenous proteins may be of relevance for the pathophysiological mechanism. Thus, modifications caused by organic acid anhydride (OAAs), persulfate and acrylates on hemoglobin (Hb) and human serum albumin (HSA) was studied in this thesis. The OAAs were found adducted to N-terminal valines and lysines. New methods using the computer softwares MSight and MoDetect were developed for identification of novel modifications by persulfate and acrylates on proteins. Persulfate oxidized tryptophans and methionines in both Hb and HSA, whereas the acrylates adducted cysteines in Hb. Liquid chromatography tandem mass spectrometry methods using selected reaction monitoring were developed for digested modified peptides, as a step toward developing methods for biological monitoring of exposure. It was shown that methylhexahydrophthalic anhydride (MHHPA) adducted peptides could be measured in nasal lavage collected from workers exposed to low levels of MHHPA and that oxidized peptides were found in digests of Hb from a subject in the reference population. It seems that the site of modification may be of importance for the effect but this has to be further evaluated.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Hälsovetenskap -- Arbetsmedicin och miljömedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Health Sciences -- Occupational Health and Environmental Health (hsv//eng)
Nyckelord
- MSight
- hemoglobin human serum albumin
- nasal lavage
- acrylates
- organic acid anhydrides
- persulfate
- biomarkers adducts
- mass spectrometry
- MoDetect
Publikations- och innehållstyp
- dok (ämneskategori)
- vet (ämneskategori)
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