Completed genome and emergence scenario of the multidrug-resistant nosocomial pathogen Staphylococcus epidermidis ST215

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Completed genome and emergence scenario of the multidrug-resistant nosocomial pathogen Staphylococcus epidermidis ST215

Kellgren, Therese (author): Umeå universitet,Institutionen för matematik och matematisk statistik

Dwibedi, Chinmay Kumar (author): Umeå universitet,Institutionen för klinisk mikrobiologi,Molekylär Infektionsmedicin, Sverige (MIMS)

Widerström, Micael, Docent, Lektor klinisk mikrobiologi, 1958- (author): Umeå universitet,Institutionen för klinisk mikrobiologi

Sundell, David (author): Division of CBRN Defence and Security, Swedish Defense Research Agency, SE, Umeå, Sweden

Öhrman, Caroline (author): Division of CBRN Defence and Security, Swedish Defense Research Agency, SE, Umeå, Sweden

Sjödin, Andreas (author): Division of CBRN Defence and Security, Swedish Defense Research Agency, SE, Umeå, Sweden

Monsen, Tor J. (author): Umeå universitet,Institutionen för klinisk mikrobiologi

Rydén, Patrik (author): Umeå universitet,Institutionen för matematik och matematisk statistik

Johansson, Anders, 1966- (author): Umeå universitet,Institutionen för klinisk mikrobiologi,Molekylär Infektionsmedicin, Sverige (MIMS)

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BioMed Central (BMC), 2024
2024
English.
In: BMC Microbiology. - : BioMed Central (BMC). - 1471-2180. ; 24:1

Related links:: https://doi.org/10.1...; show more...; https://umu.diva-por... (primary) (Raw object); https://urn.kb.se/re...; https://doi.org/10.1...; show less...

Journal article (peer-reviewed)

Abstract Subject headings

Background: A multidrug-resistant lineage of Staphylococcus epidermidis named ST215 is a common cause of prosthetic joint infections and other deep surgical site infections in Northern Europe, but is not present elsewhere. The increasing resistance among S. epidermidis strains is a global concern. We used whole-genome sequencing to characterize ST215 from healthcare settings.Results: We completed the genome of a ST215 isolate from a Swedish hospital using short and long reads, resulting in a circular 2,676,787 bp chromosome and a 2,326 bp plasmid. The new ST215 genome was placed in phylogenetic context using 1,361 finished public S. epidermidis reference genomes. We generated 10 additional short-read ST215 genomes and 11 short-read genomes of ST2, which is another common multidrug-resistant lineage at the same hospital. We studied recombination’s role in the evolution of ST2 and ST215, and found multiple recombination events averaging 30–50 kb. By comparing the results of antimicrobial susceptibility testing for 31 antimicrobial drugs with the genome content encoding antimicrobial resistance in the ST215 and ST2 isolates, we found highly similar resistance traits between the isolates, with 22 resistance genes being shared between all the ST215 and ST2 genomes. The ST215 genome contained 29 genes that were historically identified as virulence genes of S. epidermidis ST2. We established that in the nucleotide sequence stretches identified as recombination events, virulence genes were overrepresented in ST215, while antibiotic resistance genes were overrepresented in ST2.Conclusions: This study features the extensive antibiotic resistance and virulence gene content in ST215 genomes. ST215 and ST2 lineages have similarly evolved, acquiring resistance and virulence through genomic recombination. The results highlight the threat of new multidrug-resistant S. epidermidis lineages emerging in healthcare settings.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)

Keyword

Cross infection/epidemiology
Drug resistance
multiple
bacterial multidrug resistance
Healthcare-associated infections
Staphylococcus epidermidis
Whole-genome sequencing

Publication and Content Type

ref (subject category)
art (subject category)

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By the author/editor: Kellgren, Theres ...; Dwibedi, Chinmay ...; Widerström, Mica ...; Sundell, David; Öhrman, Caroline; Sjödin, Andreas; show more...; Monsen, Tor J.; Rydén, Patrik; Johansson, Ander ...; show less...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Microbiology in ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Infectious Medic ...

Articles in the publication: BMC Microbiology

By the university: Umeå University

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