WFRF:(Kubiński Konrad)
Sökning: WFRF:(Kubiński Konrad) > Yeast surviving fac...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 04007naa a2200445 4500 | |
| 001 | oai:DiVA.org:uu-16087 | |
| 003 | SwePub | |
| 008 | 080418s2008 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-160872 URI |
| 024 | 7 | a https://doi.org/10.1007/s11010-008-9721-92 DOI |
| 040 | a (SwePub)uu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Masłyk, Macieju Department of Molecular Biology, Environmental Protection Institute, John Paul II Catholic University of Lublin, Kras´nicka Av.102, 20-718 Lublin, Poland4 aut |
| 245 | 1 0 | a Yeast surviving factor Svf1 as a new interacting partner, regulator and in vitro substrate of protein kinase CK2 |
| 264 | c 2008-02-12 | |
| 264 | 1 | b Springer Science and Business Media LLC,c 2008 |
| 338 | a print2 rdacarrier | |
| 520 | a Since Svf1 is phosphoprotein, we investigated whether it was a substrate for protein kinase CK2. According to the amino acid sequence Svf1 harbours 20 putative CK2 phosphorylation sites. Here, we have reported cloning, overexpression, purification and characterization of yeast Svf1 as a substrate for three forms of yeast CK2. Svf1 serves as a substrate for both the recombinant CK2alpha (K (m) 0.35 muM) and CK2alpha' (K (m) 0.18 muM) as well as CK2 holoenzyme (K (m) 1.1 muM). Different K (m) values argue that CK2beta(beta') subunit has an inhibitory effect on the activity of both CK2alpha and CK2alpha' towards surviving factor Svf1. Reconstitution of alpha'(2)betabeta' isoform of CK2 holoenzyme shows that beta/beta' subunits have regulatory effect depending on the kind of CK2 catalytic subunit. This effect was not observed in the case of alpha(2)betabeta' isoform, which may be due to interaction between Svf1 and regulatory CK2beta subunit (shown by co-immunoprecipitation experiments). Interactions between CK2 subunits and Svf1 protein may have influence on ATP as well as ATP-competitive inhibitors (TBBt and TBBz) binding. CK2 phosphorylates up to six serine residues in highly acidic peptide K(199)EVIPESDEEESSADEDDNEDEDEESGDSEEESGSEEESDSEEVEITYED(248) of the Svf1 protein in vitro. Presented data may help to elucidate the role of protein kinase CK2 and Svf1 in the regulation of cell survival pathways. | |
| 653 | a Yeast | |
| 653 | a Protein kinase CK2 | |
| 653 | a Phosphorylation | |
| 653 | a Apoptosis | |
| 653 | a Svf1 | |
| 653 | a Mass spectrometry | |
| 653 | a Svf1–CK2b interaction | |
| 653 | a ATP-competitive inhibitors | |
| 653 | a MEDICINE | |
| 653 | a MEDICIN | |
| 700 | 1 | a Kochanowicz, Elzbietau Department of Molecular Biology, Environmental Protection Institute, John Paul II Catholic University of Lublin, Kras´nicka Av.102, 20-718 Lublin, Poland4 aut |
| 700 | 1 | a Zieliński, Rafału Department of Molecular Biology, Environmental Protection Institute, John Paul II Catholic University of Lublin, Kras´nicka Av.102, 20-718 Lublin, Poland4 aut |
| 700 | 1 | a Kubiński, Konradu Department of Molecular Biology, Environmental Protection Institute, John Paul II Catholic University of Lublin, Kras´nicka Av.102, 20-718 Lublin, Poland4 aut |
| 700 | 1 | a Hellman, Ulfu Uppsala universitet,Ludwiginstitutet för cancerforskning4 aut0 (Swepub:uu)ulfhm |
| 700 | 1 | a Szyszka, Ryszardu Department of Molecular Biology, Environmental Protection Institute, John Paul II Catholic University of Lublin, Kras´nicka Av.102, 20-718 Lublin, Poland4 aut |
| 710 | 2 | a Department of Molecular Biology, Environmental Protection Institute, John Paul II Catholic University of Lublin, Kras´nicka Av.102, 20-718 Lublin, Polandb Ludwiginstitutet för cancerforskning4 org |
| 773 | 0 | t Molecular and Cellular Biochemistryd : Springer Science and Business Media LLCg 312:1-2, s. 61-69q 312:1-2<61-69x 0300-8177x 1573-4919 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-16087 |
| 856 | 4 8 | u https://doi.org/10.1007/s11010-008-9721-9 |
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- Masłyk, Maciej
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- Zieliński, Rafał
- Kubiński, Konrad
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- Szyszka, Ryszard
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- Uppsala universitet
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