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Detection of Pseudomonas aeruginosa infection using a sustainable and selective polydopamine-based molecularly imprinted electrochemical sensor
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- Thirabowonkitphithan, Pannawich (författare)
- Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Chulalongkorn University
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- Hajizadeh, Solmaz (författare)
- Lund University,Lunds universitet,Tillämpad biokemi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,LTH profilområde: Teknik för hälsa,LTH profilområden,Pure and Applied Biochemistry,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,LTH Profile Area: Engineering Health,LTH Profile areas,Faculty of Engineering, LTH
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- Laiwattanapaisal, Wanida (författare)
- Chulalongkorn University
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- (creator_code:org_t)
- 2024
- 2024
- Engelska 11 s.
- Ingår i: European Polymer Journal. - 0014-3057. ; 209
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- Pyocyanin, a redox-active secondary metabolite produced by Pseudomonas aeruginosa, serves as a crucial virulence factor. Detection and quantification of pyocyanin can aid early diagnosis of infection. A selective and sensitive molecularly imprinted electrochemical sensor was constructed by using a green polymerization technique to deposit an ultrathin polydopamine film on an electrode modified with gold nanoparticles and chitosan. Target recognition was facilitated by specific binding sites within the imprinted polymer matrix that are complementary to the structure of pyocyanin. Various techniques, including cyclic voltammetry, electrochemical impedance spectroscopy, and square wave voltammetry, were employed to characterize the electrochemical behavior of the sensor. We investigated the influence of fabrication components, including chitosan concentration, monomer concentration, electro-polymerization conditions, pH, and rebinding time. Demonstrating a high degree of specificity and sensitivity, the sensor showcased a broad linear detection range of 1–100 µM and a low detection limit of 0.74 µM for pyocyanin. Moreover, the sensor successfully detected pyocyanin in real bacterial culture samples, exhibiting a recovery of the spiked standard ranging from 93 to 103 %. The electrochemical sensor displayed satisfactory stability lasting for at least 5 weeks. We demonstrated the sensor's applicability for clinical measurements by detecting pyocyanin in infected burn wounds using an ex vivo porcine skin model. Leveraging the synergistic advantages of molecularly imprinted polymer and the specific redox window for pyocyanin detection, the electrochemical sensor presents a promising approach for early-stage infection identification, thus contributing to enhanced treatment and more effective healthcare for patients.
Ämnesord
- NATURVETENSKAP -- Kemi -- Polymerkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Polymer Chemistry (hsv//eng)
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Miljöbioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Environmental Biotechnology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Naturresursteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Environmental Engineering (hsv//eng)
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