Characterisation of cells in regenerating cartilage from autotransplanted perichondrium. immunohistochemical expression of smooth-muscle actin, desmin, vimentin, and Ki-67.

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Fältnamn	Indikatorer	Metadata
000		02383naa a2200289 4500
001		oai:DiVA.org:uu-318507
003		SwePub
008		220116s1999 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3185072 URI
040		a (SwePub)uu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Ljung, A4 aut
245	1 0	a Characterisation of cells in regenerating cartilage from autotransplanted perichondrium. immunohistochemical expression of smooth-muscle actin, desmin, vimentin, and Ki-67.
264	1	c 1999
338		a print2 rdacarrier
520		a Autotransplanted perichondrium from rib and ear sutured to the knee joints of 26 rabbits has been examined with immunohistochemistry and shows certain structural, functional, and proliferative characteristics of regenerating cartilage. Cryostat sections have been examined for the expression of smooth-muscle actin (SMA), desmin, vimentin, and Ki-67. In this rabbit model of perichondrial grafting SMA staining showed vivid vessel regeneration, particularly in the proliferating stage about two to three weeks after grafting, and no vessels in more mature parts one month or more after transplantation. Desmin staining showed expression and distribution similar to SMA. Vimentin staining shows the cytoskeleton of regenerating cartilaginous tissue and makes cellular borders apparent. Immunohistochemical expression of Ki-67 is constantly negative in perichondrial tissue from rib and ear before transplantation, clearly positive in the proliferative stage, but there is no expression in maturing cartilage. The study also shows that all human antibodies used are applicable in a rabbit model.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicin0 (SwePub)3022 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicine0 (SwePub)3022 hsv//eng
700	1	a Ohlsén, L4 aut
700	1	a Widenfalk, B4 aut
700	1	a Gerdin, Bu Uppsala universitet,Institutionen för kirurgiska vetenskaper4 aut
710	2	a Uppsala universitetb Institutionen för kirurgiska vetenskaper4 org
773	0	t Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgeryg 33:3, s. 257-66q 33:3<257-66x 0284-4311x 1651-2073
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-318507

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Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery (Sök värdpublikationen i LIBRIS)

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Av författaren/redakt...: Ljung, A; Ohlsén, L; Widenfalk, B; Gerdin, B

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin

Artiklar i publikationen: Scandinavian Jou ...

Av lärosätet: Uppsala universitet

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