Comparative analysis of gene expression profiles between primary knee osteoarthritis and an osteoarthritis endemic to Northwestern China, Kashin-Beck disease.

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Duan, ChenMedical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China (author)

Comparative analysis of gene expression profiles between primary knee osteoarthritis and an osteoarthritis endemic to Northwestern China, Kashin-Beck disease.

Article/chapterEnglish2010

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2010-02-25
John Wiley & Sons,2010
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LIBRIS-ID:oai:DiVA.org:umu-105446
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-105446URI
https://doi.org/10.1002/art.27282DOI

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Language:English
Summary in:English

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OBJECTIVE: To investigate the differences in gene expression profiles of adult articular cartilage from patients with Kashin-Beck disease (KBD) versus those with primary knee osteoarthritis (OA).METHODS: The messenger RNA expression profiles of articular cartilage from patients with KBD, diagnosed according to the clinical criteria for KBD in China, were compared with those of cartilage from patients with OA, diagnosed according to the Western Ontario and McMaster Universities OA Index. Total RNA was isolated separately from 4 pairs of the KBD and OA cartilage samples, and the expression profiles were evaluated by Agilent 4x44k Whole Human Genome density oligonucleotide microarray analysis. The microarray data for selected transcripts were confirmed by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) amplification.RESULTS: For 1.2 x 10(4) transcripts, corresponding to 58.4% of the expressed transcripts, 2-fold changes in differential expression were revealed. Expression levels higher in KBD than in OA samples were observed in a mean + or - SD 6,439 + or - 1,041 (14.6 + or - 2.4%) of the transcripts, and expression levels were lower in KBD than in OA samples in 6,147 + or - 1,222 (14.2 + or - 2.8%) of the transcripts. After application of the selection criteria, 1.85% of the differentially expressed genes (P < 0.001 between groups) were detected. These included 233 genes, of which 195 (0.4%) were expressed at higher levels and 38 (0.08%) were expressed at lower levels in KBD than in OA cartilage. Comparisons of the quantitative RT-PCR data supported the validity of our microarray data.CONCLUSION: Differences between KBD and OA cartilage exhibited a similar pattern among all 4 of the pairs examined, indicating the presence of disease mechanisms, mainly chondrocyte matrix metabolism, cartilage degeneration, and apoptosis induction pathways, which contribute to cartilage destruction in KBD.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Ortopedi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Orthopaedics hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
NATURVETENSKAP Biologi Genetik hsv//swe
NATURAL SCIENCES Biological Sciences Genetics hsv//eng
Kashin-Beck disease
articular cartilage
gene expression profiling
cell research
cellforskning
genetik
Genetics
ortopedi
Orthopaedics
patologi
Pathology

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Guo, XiongMedical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China (author)
Zhang, Xiao-DongFirst Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China (author)
Yu, Han-JieNorthwest University, Xi'an, Shaanxi, China (author)
Yan, HuaSecond Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China (author)
Gao, YingSecond Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China (author)
Ma, Wei-JuanMedical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China (author)
Gao, Zong-QiangSecond Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China (author)
Xu, PengXi'an Red Cross Hospital, Xi'an, Shaanxi, China (author)
Lammi, Mikko,1961-University of Kuopio, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group(Swepub:umu)mila0077 (author)
Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, ChinaFirst Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China (creator_code:org_t)

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