Comparative analysis of gene expression profiles between primary knee osteoarthritis and an osteoarthritis endemic to Northwestern China, Kashin-Beck disease.

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Fältnamn	Indikatorer	Metadata
000		05262naa a2200601 4500
001		oai:DiVA.org:umu-105446
003		SwePub
008		150623s2010 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1054462 URI
024	7	a https://doi.org/10.1002/art.272822 DOI
040		a (SwePub)umu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Duan, Chenu Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China4 aut
245	1 0	a Comparative analysis of gene expression profiles between primary knee osteoarthritis and an osteoarthritis endemic to Northwestern China, Kashin-Beck disease.
264		c 2010-02-25
264	1	b John Wiley & Sons,c 2010
338		a print2 rdacarrier
520		a OBJECTIVE: To investigate the differences in gene expression profiles of adult articular cartilage from patients with Kashin-Beck disease (KBD) versus those with primary knee osteoarthritis (OA).METHODS: The messenger RNA expression profiles of articular cartilage from patients with KBD, diagnosed according to the clinical criteria for KBD in China, were compared with those of cartilage from patients with OA, diagnosed according to the Western Ontario and McMaster Universities OA Index. Total RNA was isolated separately from 4 pairs of the KBD and OA cartilage samples, and the expression profiles were evaluated by Agilent 4x44k Whole Human Genome density oligonucleotide microarray analysis. The microarray data for selected transcripts were confirmed by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) amplification.RESULTS: For 1.2 x 10(4) transcripts, corresponding to 58.4% of the expressed transcripts, 2-fold changes in differential expression were revealed. Expression levels higher in KBD than in OA samples were observed in a mean + or - SD 6,439 + or - 1,041 (14.6 + or - 2.4%) of the transcripts, and expression levels were lower in KBD than in OA samples in 6,147 + or - 1,222 (14.2 + or - 2.8%) of the transcripts. After application of the selection criteria, 1.85% of the differentially expressed genes (P < 0.001 between groups) were detected. These included 233 genes, of which 195 (0.4%) were expressed at higher levels and 38 (0.08%) were expressed at lower levels in KBD than in OA cartilage. Comparisons of the quantitative RT-PCR data supported the validity of our microarray data.CONCLUSION: Differences between KBD and OA cartilage exhibited a similar pattern among all 4 of the pairs examined, indicating the presence of disease mechanisms, mainly chondrocyte matrix metabolism, cartilage degeneration, and apoptosis induction pathways, which contribute to cartilage destruction in KBD.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Ortopedi0 (SwePub)302112 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Orthopaedics0 (SwePub)302112 hsv//eng
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng
650	7	a NATURVETENSKAPx Biologix Genetik0 (SwePub)106092 hsv//swe
650	7	a NATURAL SCIENCESx Biological Sciencesx Genetics0 (SwePub)106092 hsv//eng
653		a Kashin-Beck disease
653		a articular cartilage
653		a gene expression profiling
653		a cell research
653		a cellforskning
653		a genetik
653		a Genetics
653		a ortopedi
653		a Orthopaedics
653		a patologi
653		a Pathology
700	1	a Guo, Xiongu Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China4 aut
700	1	a Zhang, Xiao-Dongu First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China4 aut
700	1	a Yu, Han-Jieu Northwest University, Xi'an, Shaanxi, China4 aut
700	1	a Yan, Huau Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China4 aut
700	1	a Gao, Yingu Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China4 aut
700	1	a Ma, Wei-Juanu Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, China4 aut
700	1	a Gao, Zong-Qiangu Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China4 aut
700	1	a Xu, Pengu Xi'an Red Cross Hospital, Xi'an, Shaanxi, China4 aut
700	1	a Lammi, Mikko,d 1961-u University of Kuopio, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group4 aut0 (Swepub:umu)mila0077
710	2	a Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, Chinab First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, China4 org
773	0	t Arthritis and Rheumatismd : John Wiley & Sonsg 62:3, s. 771-780q 62:3<771-780x 0004-3591x 1529-0131
856	4	u http://onlinelibrary.wiley.com/doi/10.1002/art.27282/abstracty Article Web page
856	4	u https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/art.27282
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-105446
856	4 8	u https://doi.org/10.1002/art.27282

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