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Comprehensive prote...
Comprehensive proteome analysis of nasal lavage samples after controlled exposure to welding nanoparticles shows an induced acute phase and a nuclear receptor, LXR/RXR, activation that influence the status of the extracellular matrix
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- Ali, Neserin (författare)
- Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine,Lund Univ, Sweden
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- Ljunggren, Stefan, 1988- (författare)
- Linköpings universitet,Linköping University,Avdelningen för neuro- och inflammationsvetenskap,Medicinska fakulteten,Region Östergötland, Arbets- och miljömedicin
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- Karlsson, Helen (författare)
- Linköpings universitet,Linköping University,Avdelningen för neuro- och inflammationsvetenskap,Medicinska fakulteten,Region Östergötland, Arbets- och miljömedicin
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visa fler...
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- Wierzbicka, Aneta (författare)
- Lund University,Lunds universitet,Ergonomi och aerosolteknologi,Institutionen för designvetenskaper,Institutioner vid LTH,Lunds Tekniska Högskola,Ergonomics and Aerosol Technology,Department of Design Sciences,Departments at LTH,Faculty of Engineering, LTH,Lund Univ, Sweden
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- Pagels, Joakim (författare)
- Lund University,Lunds universitet,Ergonomi och aerosolteknologi,Institutionen för designvetenskaper,Institutioner vid LTH,Lunds Tekniska Högskola,Ergonomics and Aerosol Technology,Department of Design Sciences,Departments at LTH,Faculty of Engineering, LTH,Lund Univ, Sweden
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- Isaxon, Christina (författare)
- Lund University,Lunds universitet,Ergonomi och aerosolteknologi,Institutionen för designvetenskaper,Institutioner vid LTH,Lunds Tekniska Högskola,Ergonomics and Aerosol Technology,Department of Design Sciences,Departments at LTH,Faculty of Engineering, LTH,Lund Univ, Sweden
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- Gudmundsson, Anders (författare)
- Lund University,Lunds universitet,Ergonomi och aerosolteknologi,Institutionen för designvetenskaper,Institutioner vid LTH,Lunds Tekniska Högskola,Ergonomics and Aerosol Technology,Department of Design Sciences,Departments at LTH,Faculty of Engineering, LTH,Lund Univ, Sweden
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- Rissler, Jenny (författare)
- Lund University,Lunds universitet,Ergonomi och aerosolteknologi,Institutionen för designvetenskaper,Institutioner vid LTH,Lunds Tekniska Högskola,Ergonomics and Aerosol Technology,Department of Design Sciences,Departments at LTH,Faculty of Engineering, LTH,Lund Univ, Sweden
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- Nielsen, Jörn (författare)
- Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine,Lund Univ, Sweden
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- Lindh, Christian H. (författare)
- Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine,Lund Univ, Sweden
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- Kåredal, Monica (författare)
- Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine,Lund Univ, Sweden
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(creator_code:org_t)
- 2018-05-11
- 2018
- Engelska.
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Ingår i: Clinical Proteomics. - : Springer Science and Business Media LLC. - 1542-6416 .- 1559-0275. ; 15:1
- Relaterad länk:
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http://dx.doi.org/10... (free)
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visa fler...
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https://clinicalprot...
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https://liu.diva-por... (primary) (Raw object)
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Background: Epidemiological studies have shown that many welders experience respiratory symptoms. During the welding process a large number of airborne nanosized particles are generated, which might be inhaled and deposited in the respiratory tract. Knowledge of the underlying mechanisms behind observed symptoms is still partly lacking, although inflammation is suggested to play a central role. The aim of this study was to investigate the effects of welding fume particle exposure on the proteome expression level in welders suffering from respiratory symptoms, and changes in protein mediators in nasal lavage samples were analyzed. Such mediators will be helpful to clarify the pathomechanisms behind welding fume particle-induced effects. Methods: In an exposure chamber, 11 welders with work-related symptoms in the lower airways during the last month were exposed to mild-steel welding fume particles (1 mg/m3) and to filtered air, respectively, in a double-blind manner. Nasal lavage samples were collected before, immediately after, and the day after exposure. The proteins in the nasal lavage were analyzed with two different mass spectrometry approaches, label-free discovery shotgun LC-MS/MS and a targeted selected reaction monitoring LC-MS/MS analyzing 130 proteins and four in vivo peptide degradation products. Results: The analysis revealed 30 significantly changed proteins that were associated with two main pathways; activation of acute phase response signaling and activation of LXR/RXR, which is a nuclear receptor family involved in lipid signaling. Connective tissue proteins and proteins controlling the degradation of such tissues, including two different matrix metalloprotease proteins, MMP8 and MMP9, were among the significantly changed enzymes and were identified as important key players in the pathways. Conclusion: Exposure to mild-steel welding fume particles causes measurable changes on the proteome level in nasal lavage matrix in exposed welders, although no clinical symptoms were manifested. The results suggested that the exposure causes an immediate effect on the proteome level involving acute phase proteins and mediators regulating lipid signaling. Proteases involved in maintaining the balance between the formation and degradation of extracellular matrix proteins are important key proteins in the induced effects.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Lungmedicin och allergi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Respiratory Medicine and Allergy (hsv//eng)
Nyckelord
- Chamber study
- Effects
- Mass spectrometry
- Nasal lavage
- Pathways
- Proteomics
- Welding fume particles
- Chamber study; Welding fume particles; Nasal lavage; Effects; Proteomics; Mass spectrometry; Pathways
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Ali, Neserin
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Ljunggren, Stefa ...
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Karlsson, Helen
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Wierzbicka, Anet ...
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Pagels, Joakim
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Isaxon, Christin ...
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visa fler...
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Gudmundsson, And ...
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Rissler, Jenny
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Nielsen, Jörn
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Lindh, Christian ...
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Kåredal, Monica
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Lungmedicin och ...
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Clinical Proteom ...
- Av lärosätet
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Lunds universitet
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Linköpings universitet