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  • Spinozzi, DanieleNetherlands Inst Innovat Ocular Surg, Netherlands (författare)

Evaluation of the Suitability of Biocompatible Carriers as Artificial Transplants Using Cultured Porcine Corneal Endothelial Cells

  • Artikel/kapitelEngelska2019

Förlag, utgivningsår, omfång ...

  • 2018-12-07
  • TAYLOR & FRANCIS INC,2019
  • electronicrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:liu-155534
  • https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-155534URI
  • https://doi.org/10.1080/02713683.2018.1536215DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Funding Agencies|European Unions Horizon 2020 research and innovation programme [667400]
  • Purpose/Aim: Evaluating the suitability of bioengineered collagen sheets and human anterior lens capsules (HALCs) as carriers for cultivated porcine corneal endothelial cells (pCECs) and in vitro assessment of the cell-carrier sheets as tissue-engineered grafts for Descemet membrane endothelial keratoplasty (DMEK). Materials and Methods: pCECs were isolated, cultured up to P2 and seeded onto LinkCell (TM) bioengineered matrices of 20 mu m (LK20) or 100 mu m (LK100) thickness, and on HALC. During expansion, pCEC viability and morphology were assessed by light microscopy. ZO-1 and Na+/K+-ATPase expression was investigated by immunohistochemistry. Biomechanical properties of pCEC-carrier constructs were evaluated by simulating DMEK surgery in vitro using an artificial anterior chamber (AC) and a human donor cornea without Descemet membrane (DM). Results: During in vitro expansion, cultured pCECs retained their proliferative capacity, as shown by the positive staining for proliferative marker Ki67, and a high cell viability rate (96 +/- 5%). pCECs seeded on all carriers formed a monolayer of hexagonal, tightly packed cells that expressed ZO-1 and Na+/K+-ATPase. During in vitro surgery, pCEC-LK20 and pCEC-LK100 constructs were handled like Descemet stripping endothelial keratoplasty (DSEK) grafts, i.e. folded like a "taco" for insertion because of challenges related to rolling and sticking of the grafts in the injector. pCEC-HALC constructs behaved similar to the DMEK reference model during implantation and unfolding in the artificial AC, showing good adhesion to the bare stroma. Conclusions: In vitro DMEK surgery showed HALC as the most suitable carrier for cultivated pCECs with good intraoperative graft handling. LK20 carrier showed good biocompatibility, but required a DSEK-adapted surgical protocol. Both carriers might be notional candidates for potential future clinical applications.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Miron, AlinaNetherlands Inst Innovat Ocular Surg, Netherlands (författare)
  • Bruinsma, MariekeNetherlands Inst Innovat Ocular Surg, Netherlands (författare)
  • Dapena, IsabelNetherlands Inst Innovat Ocular Surg, Netherlands; Melles Cornea Clin Rotterdam, Netherlands (författare)
  • Lavy, ItayNetherlands Inst Innovat Ocular Surg, Netherlands; Melles Cornea Clin Rotterdam, Netherlands (författare)
  • Binder, Perry S.Univ Calif Irvine, CA USA (författare)
  • Rafat, MehrdadLinköpings universitet,Avdelningen för medicinsk teknik,Tekniska fakulteten,LinkoCare Life Sci AB, Linkoping, Sweden(Swepub:liu)mehra64 (författare)
  • Oellerich, SilkeNetherlands Inst Innovat Ocular Surg, Netherlands (författare)
  • Melles, Gerrit R. J.Netherlands Inst Innovat Ocular Surg, Netherlands; Melles Cornea Clin Rotterdam, Netherlands; Amnitrans EyeBank Rotterdam, Netherlands (författare)
  • Netherlands Inst Innovat Ocular Surg, NetherlandsNetherlands Inst Innovat Ocular Surg, Netherlands; Melles Cornea Clin Rotterdam, Netherlands (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Current Eye Research: TAYLOR & FRANCIS INC44:3, s. 243-2490271-36831460-2202

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