Upregulation of GADD45a, TP53 and CASP3 mRNA expression in the rat lens after in vivo exposure to sub-threshold exposure to UVR B

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Fältnamn	Indikatorer	Metadata
000		03055naa a2200409 4500
001		oai:DiVA.org:uu-232624
003		SwePub
008		140922s2014 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2326242 URI
040		a (SwePub)uu
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Galichanin, Konstantinu Uppsala universitet,Oftalmiatrik,Gullstrand lab/ Leader Per Söderberg4 aut0 (Swepub:uu)konga695
245	1 0	a Upregulation of GADD45a, TP53 and CASP3 mRNA expression in the rat lens after in vivo exposure to sub-threshold exposure to UVR B
264	1	c 2014
338		a print2 rdacarrier
520		a Purpose:The objective of the present study was to investigate the evolution of mRNA expression of the stress sensor GADD45α, the apoptosis initiator TP53 and the apoptosis executor CASP3 in the rat lens after in vivo exposure to sub-threshold dose of UVR-B.Methods:Forty six-week-old female albino Sprague-Dawley rats were unilaterally exposed to a sub-threshold dose, 1 kJ/m2 (1.1 W/m2 for 15 min), of UVR (λmax = 300 nm). Anesthetized and dilated eyes were exposed to UVB radiation. The animals were sacrificed at 1, 5, 24 and 120 h post-exposure. mRNA expression of the GADD45α, TP53 and CASP3 genes in the lenses was measured by quantitative RT‑PCR, and fold change in mRNA expression between exposed and unexposed lenses was calculated.Results:mRNA expression for GADD45α increased to a 1.2 fold change at 1 h after exposure and then returned to no change at 120 h. mRNA expression for TP53 increased with a regression coefficient of 0.04 h-1 to a maximum of 1.67 fold change. mRNA expression for CASP3 increased with a regression coefficient of 4.5 x10-3 rel. units/h to a 1.46 fold change at 120 h after exposure.Conclusions:A sub-threshold in vivo exposure to UVR-B causes a transient upregulation of the stress sensor GADD45α at 1 h after exposure, a saturating upregulation of TP53 and a subsequent constant upregulation of CASP3 in the rat lens.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Oftalmologi0 (SwePub)302172 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Ophthalmology0 (SwePub)302172 hsv//eng
653		a Lens
653		a UVR
653		a Sub-threshold
653		a Dose
653		a GADD45a
653		a TP53
653		a CASP3
653		a Apoptosis
653		a Ophtalmology
653		a Oftalmiatrik
700	1	a Yu, Zhaohua,d 1983-u Uppsala universitet,Oftalmiatrik,Gullstrand lab/ Leader Per Söderberg4 aut0 (Swepub:uu)zhayu760
700	1	a Söderberg, Peru Uppsala universitet,Oftalmiatrik4 aut0 (Swepub:uu)perso438
710	2	a Uppsala universitetb Oftalmiatrik4 org
773	0	t Journal of Ocular Biologyg 2:1, s. 5-q 2:1<5-x 2334-2838
856	4	u http://www.avensonline.org/wp-content/uploads/2014/08/JOCB-2334-2838-02-0016.pdf
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-232624

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Av författaren/redakt...: Galichanin, Kons ...; Yu, Zhaohua, 198 ...; Söderberg, Per

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Oftalmologi

Artiklar i publikationen: Journal of Ocula ...

Av lärosätet: Uppsala universitet

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