Patterns of Transcript Abundance of Eukaryotic Biogeochemically-Relevant Genes in the Amazon River Plume

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Sökning: (WFRF:(Byron John)) > Patterns of Transcr...

LIBRIS Formathandbok (Information om MARC21)

Fältnamn	Indikatorer	Metadata
000		04694naa a2200517 4500
001		oai:DiVA.org:su-135961
003		SwePub
008		161128s2016 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-1359612 URI
024	7	a https://doi.org/10.1371/journal.pone.01609292 DOI
040		a (SwePub)su
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Zielinski, Brian L.4 aut
245	1 0	a Patterns of Transcript Abundance of Eukaryotic Biogeochemically-Relevant Genes in the Amazon River Plume
264		c 2016-09-06
264	1	b Public Library of Science (PLoS),c 2016
338		a print2 rdacarrier
520		a The Amazon River has the largest discharge of all rivers on Earth, and its complex plume system fuels a wide array of biogeochemical processes, across a large area of the western tropical North Atlantic. The plume thus stimulates microbial processes affecting carbon sequestration and nutrient cycles at a global scale. Chromosomal gene expression patterns of the 2.0 to 156 mu m size-fraction eukaryotic microbial community were investigated in the Amazon River Plume, generating a robust dataset (more than 100 million mRNA sequences) that depicts the metabolic capabilities and interactions among the eukaryotic microbes. Combining classical oceanographic field measurements with metatranscriptomics yielded characterization of the hydrographic conditions simultaneous with a quantification of transcriptional activity and identity of the community. We highlight the patterns of eukaryotic gene expression for 31 biogeochemically significant gene targets hypothesized to be valuable within forecasting models. An advantage to this targeted approach is that the database of reference sequences used to identify the target genes was selectively constructed and highly curated optimizing taxonomic coverage, throughput, and the accuracy of annotations. A coastal diatom bloom highly expressed nitrate transporters and carbonic anhydrase presumably to support high growth rates and enhance uptake of low levels of dissolved nitrate and CO2. Diatom-diazotroph association (DDA: diatoms with nitrogen fixing symbionts) blooms were common when surface salinity was mesohaline and dissolved nitrate concentrations were below detection, and hence did not show evidence of nitrate utilization, suggesting they relied on ammonium transporters to aquire recently fixed nitrogen. These DDA blooms in the outer plume had rapid turnover of the photosystem D1 protein presumably caused by photodegradation under increased light penetration in clearer waters, and increased expression of silicon transporters as silicon became limiting. Expression of these genes, including carbonic anhydrase and transporters for nitrate and phosphate, were found to reflect the physiological status and biogeochemistry of river plume environments. These relatively stable patterns of eukaryotic transcript abundance occurred over modest spatiotemporal scales, with similarity observed in sample duplicates collected up to 2.45 km in space and 120 minutes in time. These results confirm the use of metatranscriptomics as a valuable tool to understand and predict microbial community function.
650	7	a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe
650	7	a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng
700	1	a Allen, Andrew E.4 aut
700	1	a Carpenter, Edward J.4 aut
700	1	a Coles, Victoria J.4 aut
700	1	a Crump, Byron C.4 aut
700	1	a Doherty, Mary4 aut
700	1	a Foster, Rachel A.u Stockholms universitet,Institutionen för ekologi, miljö och botanik,University of California, USA4 aut0 (Swepub:su)rfost
700	1	a Goes, Joaquim I.4 aut
700	1	a Gomes, Helga R.4 aut
700	1	a Hood, Raleigh R.4 aut
700	1	a McCrow, John P.4 aut
700	1	a Montoya, Joseph P.4 aut
700	1	a Moustafa, Ahmed4 aut
700	1	a Satinsky, Brandon M.4 aut
700	1	a Sharma, Shalabh4 aut
700	1	a Smith, Christa B.4 aut
700	1	a Yager, Patricia L.4 aut
700	1	a Paul, John H.4 aut
710	2	a Stockholms universitetb Institutionen för ekologi, miljö och botanik4 org
773	0	t PLOS ONEd : Public Library of Science (PLoS)g 11:9q 11:9x 1932-6203
856	4	u https://doi.org/10.1371/journal.pone.0160929y Fulltext
856	4	u https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0160929&type=printable
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-135961
856	4 8	u https://doi.org/10.1371/journal.pone.0160929

Hitta via bibliotek

PLOS ONE (Sök värdpublikationen i LIBRIS)

Till lärosätets databas

Hitta mer i SwePub

Om ämnet

NATURVETENSKAP: NATURVETENSKAP; och Biologi

Artiklar i publikationen: PLOS ONE

Av lärosätet: Stockholms universitet

Sök utanför SwePub

Sök vidare i:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se