(WFRF:(Guo W))
Search: (WFRF:(Guo W)) > (2000-2004) > Stimulatory activit...
- 5 of 6
- Previous record
- Next record
- To hitlist
Stimulatory activity of anti-peptide antibodies against the second extracellular loop of human M2 muscarinic receptors.
- Wang, W (author)
- Guo, G (author)
- Tang, J (author)
- show more...
- Li, J (author)
- Zhao, R (author)
- show less...
- (creator_code:org_t)
- 2000
- 2000
- English.
- In: Chinese medical journal. - 0366-6999. ; 113:10, s. 867-71
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- OBJECTIVE: To study the activity of anti-peptide antibodies against the second extracellular loop of human M2 muscarinic receptors on cAMP production and inward calcium currents (Ica) in guinea pig ventricular myocytes. A comparison was also made with those of a muscarinic receptor agonist. METHODS: cAMP content was determined by radioimmunoassay and the Ica in guinea pig single ventricular cells were recorded by the whole-cell patch clamp technique. RESULTS: Both the muscarinic receptor agonist, carbachol (Carb 10 mumol/L), and anti-peptide antibodies (Abs 100 nmol/L) could decrease basal cAMP levels (by 46.9% +/- 4.2% and 60.2% +/- 4.6%, respectively) and basal Ica. Both Carb (10 mumol/L) and Abs (100 nmol/L) could also inhibit the isoprenaline-induced (Iso 0.8 mumol/L) increases in cAMP production (from 108.2 +/- 7.0 to 88.4 +/- 7.2 pmol/mg.protein/min for Carb and 88.6 +/- 5.1 pmol/mg.protein/min for Abs, respectively) and the increases in Ica. The muscarinic receptor antagonist atropine (Atr) was able to prevent these effects of Carb and Abs. CONCLUSIONS: Anti-peptide antibodies against an epitope located in the second extracellular loop of human M2 muscarinic receptors, similar to muscarinic receptor agonist, could decrease the basal Ica and beta-receptor agonist stimulated increase of Ica by decreasing the basal and beta-receptor agonist stimulated increase of cAMP production, and therefore could have an effect on their target receptor. These results further suggest that autoimmunity may participate in the pathogenesis of human cardiomyopathy and the second extracellular loop of human M2 muscarinic receptor could be the main immunodominant region.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Keyword
- Amino Acid Sequence
- Animals
- Antibodies
- immunology
- Atropine
- pharmacology
- Calcium
- metabolism
- Carbachol
- pharmacology
- Cyclic AMP
- biosynthesis
- Female
- Guinea Pigs
- Humans
- Male
- Molecular Sequence Data
- Peptide Fragments
- immunology
- Receptor
- Muscarinic M2
- Receptors
- Muscarinic
- immunology
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Chinese medical journal (Search for host publication in LIBRIS)
To the university's database
- 5 of 6
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Wang, W
- Guo, G
- Tang, J
- Li, J
- Zhao, R
- Hjalmarson, Åke, ...
- show more...
- Fu, Michael, 196 ...
- show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Microbiology in ...
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Cell and Molecul ...
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Physiology
- Articles in the publication
- Chinese medical ...
- By the university
- University of Gothenburg
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
