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Cadmium uptake and interaction with phytochelatins in wheat protoplasts : Cadmium uptake and interaction with phytochelatins in wheat protoplasts
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- Lindberg, Sylvia (author)
- Stockholms universitet,Botaniska institutionen,växtfysiologi,Sylvia Lindberg
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- Landberg, Tommy (author)
- Stockholms universitet,Botaniska institutionen,växtfysiologi,Maria Greger
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- Greger, Maria (author)
- Stockholms universitet,Botaniska institutionen,växtfysiologi,Maria greger
- (creator_code:org_t)
- 2007
- 2007
- English.
- In: Plant Physiology and Biochemistry: Plant Physiology and Biochemistry. ; 45:1, s. 47-53
- Journal article (peer-reviewed)
Abstract
Subject headings
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- In order to investigate the role of phytochelatins in short-time uptake of Cd2+ into the cytosol of wheat protoplasts, a new method wasapplied, using fluorescence microscopy and the heavy metal-specific fluorescent dye, 5-nitrobenzothiazole coumarin, BTC-5N. The uptake of Cd2+ into protoplasts from 5- to 7-day-old wheat seedlings (Triticum aestivum, L. cv. Kadett) was lower in protoplasts from seedlings raised inthe presence of 1 mM CdCl2, than in the absence. Presence of CdCl2 in the cultivation medium increased the content of phytochelatins (PCs) in the protoplasts. When seedlings were raised in the presence of both Cd2+ and buthionine sulfoximine (BSO), an inhibitor of glutathione (GSH) synthesis, only little PC was found in the protoplasts. Pre-treatment with BSO alone did not affect the content of PC, but inhibited that of GSH. The inhibition of GSH was independent of pre-treatment with Cd2+. Unidirectional flux analyses, using 109Cd2+, showed approximately the same uptake pattern of Cd2+ as did the fluorescence experiments showing the cytosolic uptake of Cd2+. Thus, the diminished uptake of Cd2+ into protoplasts from cadmium-pre-treated plants was not depending on PCs. Instead, it is likely that pre-treatment with Cd2+ causes a down-regulation of the short-term Cd2+ uptake, or an up-regulation of the Cd2+ extrusion. Moreover, since addition of Cd2+ to protoplasts from control plants caused a cytosol acidification, it is likely that a Cd2+/H+-antiport mechanism is involved in the extrusion of Cd2+ from these protoplasts.
Subject headings
- NATURVETENSKAP -- Biologi -- Botanik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Botany (hsv//eng)
Keyword
- Cadmium uptake
- Fluorescence microscopy
- Protoplasts
- Phytochelatins
- Triticum aestivum
- Plant physiology
- Växtfysiologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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