L773:0271 3683
Sökning: L773:0271 3683 > Xenobiotic- and Ser...
- Björkblom, BennyUmeå universitet,Kemiska institutionen (författare)
Xenobiotic- and Serum-Free Culture of Oral Mucosal Epithelial Cells on Contact Lenses
- Artikel/kapitelEngelska2016
Förlag, utgivningsår, omfång ...
- 2015-02-06
- Informa UK Limited,2016
- printrdacarrier
Nummerbeteckningar
- LIBRIS-ID:oai:DiVA.org:umu-114566
- https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-114566URI
- https://doi.org/10.3109/02713683.2015.1004720DOI
Kompletterande språkuppgifter
- Språk:engelska
- Sammanfattning på:engelska
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- SwePubSwePub
Klassifikation
Anmärkningar
- Purpose/aim: Cultured autologous oral mucosal epithelial cells (OMECs) have proven useful in the treatment of ocular surface disorders. This study is the first to investigate the potential of expanding OMEC in a xenobiotic- and serum-free medium using therapeutic contact lenses (CLs) as a substrate and carrier.Materials and methods: Porcine OMEC were seeded on laminin-coated lotrafilcon A therapeutic CLs with the density of 8x10(4)cells/lens and cultured in a defined serum and xenobiotic-free medium. Confocal immunofluorescence microscopy was used to analyze the following: (1) cellular morphology by using rhodamine-phalloidin staining of F-actin, (2) phenotype by applying antibodies against the progenitor cell marker p63 and the putative stem cell marker ABCG2 and (3) cell viability by using propidium iodide and Hoechst 33342 dual staining.Results: Porcine OMEC attached well to the CLs, and cell-to-cell contacts were evident. After three days in culture, the OMEC displayed a confluent monolayer with uniform cobblestone morphology, whereas stratified cultures with 2-3 layers were formed after six days. No significant difference in expression of p63 was observed after three-day culture (79.414.8%) compared with six-day culture (60.3 +/- 18.9%). ABCG2 expression in the basal cell layer was 6.3 +/- 1.0% and 4.8 +/- 1.8% after three- and six-day culture, respectively. The basal layer viability of cultured OMECs was 99.3 +/- 0.2% and 82.8 +/- 1.1% after three and six days culture, respectively.Conclusions: The use of therapeutic CLs has potential as a substrate and carrier for OMEC cultured in a xenobiotic- and serum-free culture system.
Ämnesord och genrebeteckningar
- MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Oftalmologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Clinical Medicine Ophthalmology hsv//eng
- Cell culture
- oral mucosa
- contact lens
- epithelial cells
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Eidet, Jon R. (författare)
- Utheim, Tor P. (författare)
- Ulltveit-Moe, Harald F. (författare)
- Raeder, Sten (författare)
- Umeå universitetKemiska institutionen (creator_code:org_t)
Sammanhörande titlar
- Ingår i:Current Eye Research: Informa UK Limited41:1, s. 20-270271-36831460-2202
Internetlänk
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- Av författaren/redakt...
- Björkblom, Benny
- Eidet, Jon R.
- Utheim, Tor P.
- Ulltveit-Moe, Ha ...
- Raeder, Sten
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
- MEDICIN OCH HÄLS ...
- och Klinisk medicin
- och Oftalmologi
- Artiklar i publikationen
- Current Eye Rese ...
- Av lärosätet
- Umeå universitet
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