L773:1438 4221
Sökning: L773:1438 4221 > Molecular typing of...
- Kolak, M.Karolinska Institutet (författare)
Molecular typing of the bacterial flora in sputum of cystic fibrosis patients
- Artikel/kapitelEngelska2003
Förlag, utgivningsår, omfång ...
- Elsevier BV,2003
- printrdacarrier
Nummerbeteckningar
- LIBRIS-ID:oai:DiVA.org:liu-46534
- https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-46534URI
- https://doi.org/10.1078/1438-4221-00265DOI
- http://kipublications.ki.se/Default.aspx?queryparsed=id:1944189URI
Kompletterande språkuppgifter
- Språk:engelska
- Sammanfattning på:engelska
Ingår i deldatabas
- SwePubSwePub
Klassifikation
Anmärkningar
- Despite recent advances in therapy, lower airway infections remain the major cause of morbidity and mortality in cystic fibrosis (CF) patients. Bacterial colonisation of the lower airways in CF is limited to a few bacterial species, commonly Staphylococcus aureus, Pseudomonas aeruginosa and Haemophilus influenzae. Burkholderia cepacia colonisation is much rarer, but it has been thought to be associated with more advanced lung disease and increased mortality. A rapid characterisation of the bacterial flora in sputum of CF patients is of great importance for proper treatment. The aim of this study was to establish bacterial profiles and to identify pathogenic bacteria in respiratory specimens by means of molecular methods including temporal temperature gradient gel electrophoresis (TTGE) and DNA sequencing of PCR amplicons derived from 16S rDNA variable V3 and V6 regions. Sputa of 13 CF patients (7 males/6 females, age 19-59 years) collected at the Stockholm CF centre were analysed. TTGE revealed the presence of complex bacterial profiles in all samples. The V3 and V6 PCR amplicons were cloned and sequenced by real-time DNA PyrosequencingTM. DNA from Staphylococcus aureus, Haemophilus influenzae, and Pseudomonas aeruginosa, respectively, was identified together with sequences from normal oral cavity flora. The results were in reasonable agreement with those obtained by conventional bacterial culture, considering that only known CF pathogens are included in routine reports. However, the methodology seems too elaborate to be introduced into daily routine.
Ämnesord och genrebeteckningar
- 16S rRNA
- Bacterial typing
- Cystic fibrosis
- Pyrosequencing
- Sputum
- Temporal temperature gradient gel electrophoresis
- MEDICINE
- MEDICIN
Biuppslag (personer, institutioner, konferenser, titlar ...)
- Karpati, F.Karolinska Institutet (författare)
- Monstein, Hans-JurgÖstergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Klinisk mikrobiologi,Molekylärbiologiska tekniklaboratoriet(Swepub:liu)hanmo19 (författare)
- Jonasson, JonÖstergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Molekylär och immunologisk patologi,Klinisk patologi och klinisk genetik(Swepub:liu)jonjo59 (författare)
- Karolinska InstitutetHälsouniversitetet (creator_code:org_t)
Sammanhörande titlar
- Ingår i:International Journal of Medical Microbiology: Elsevier BV293:4, s. 309-3171438-42211618-0607
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- International Journal of Medical Microbiology (Sök värdpublikationen i LIBRIS)
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- Av författaren/redakt...
- Kolak, M.
- Karpati, F.
- Monstein, Hans-J ...
- Jonasson, Jon
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- Linköpings universitet
- Karolinska Institutet
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