Persistent glucose transporter expression on pancreatic beta cells from longstanding type 1 diabetic individuals

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Coppieters, Ken T.La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA 92037 USA (författare)

Persistent glucose transporter expression on pancreatic beta cells from longstanding type 1 diabetic individuals

Artikel/kapitelEngelska2011

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2011-11-08
Wiley,2011
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LIBRIS-ID:oai:DiVA.org:uu-304613
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-304613URI
https://doi.org/10.1002/dmrr.1246DOI

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Språk:engelska
Sammanfattning på:engelska

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BACKGROUND: Recent reports have established the notion that many patients with longstanding type 1 diabetes (T1D) possess a remnant population of insulin-producing beta cells. It remains questionable, however, whether these surviving cells can physiologically sense and respond to glucose stimuli.METHODS: Frozen pancreatic sections from non-diabetic donors (n=8), type 2 diabetic patients (n=4), islet autoantibody-positive non-diabetic patients (n=3), type 1 diabetic patients (n=10) and one case of gestational diabetes were obtained via the network for Pancreatic Organ Donors. All longstanding T1D samples were selected based on the detection of insulin-producing beta cells in the pancreas by immunohistochemistry. RNA was isolated from all sections followed by cDNA preparation and quantitative real-time polymerase chain reaction for insulin, glucose transporter 1 (GLUT1), GLUT2 and GLUT3. Finally, immunofluorescent staining was performed on consecutive sections for all four of these markers and a comparison was made between the expression of GLUT2 in humans versus NOD mice.RESULTS: In contrast to islets from the most widely used T1D model, the NOD mouse, human islets predominantly express GLUT1 and, to a much lesser extent, GLUT3 on their surface instead of GLUT2. Relative expression levels of these receptors do not significantly change in the context of the various (pre-)diabetic conditions studied. Moreover, in both species preservation of GLUT expression was observed even under conditions of substantial leucocyte infiltration or decades of T1D duration.CONCLUSIONS: These data suggest that despite being subjected to multiple years of physiological stress, the remaining beta-cell population in longstanding T1D patients retains a capacity to sense glucose via its GLUTs.

Ämnesord och genrebeteckningar

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Klinisk laboratoriemedicin hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Clinical Laboratory Medicine hsv//eng
glucose transporters; type 1 diabetes; beta cells; pancreas; islets of Langerhans; insulin

Biuppslag (personer, institutioner, konferenser, titlar ...)

Wiberg, AnnaLa Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA 92037 USA(Swepub:uu)annwi478 (författare)
Amirian, NatalieLa Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA 92037 USA (författare)
Kay, Thomas W.St Vincents Inst, Dept Immunol & Diabet, Melbourne, Vic 3065, Australia (författare)
von Herrath, Matthias G.La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA 92037 USA (författare)
La Jolla Inst Allergy & Immunol, Type Diabet Ctr 1, La Jolla, CA 92037 USASt Vincents Inst, Dept Immunol & Diabet, Melbourne, Vic 3065, Australia (creator_code:org_t)

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Ingår i:Diabetes/Metabolism Research Reviews: Wiley27:8, s. 746-7541520-75521520-7560

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Av författaren/redakt...: Coppieters, Ken ...; Wiberg, Anna; Amirian, Natalie; Kay, Thomas W.; von Herrath, Mat ...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Klinisk laborato ...

Artiklar i publikationen: Diabetes/Metabol ...

Av lärosätet: Uppsala universitet

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