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Surface-enhanced Ra...
Surface-enhanced Raman scattering imaging of single living lymphocytes with multivariate evaluation
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- Eliasson, Charlotte, 1973 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Lorén, Anders, 1974 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Engelbrektsson, Johan, 1976 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Josefson, Mats (författare)
- AstraZeneca AB
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- Abrahamsson, Jonas, 1954 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kvinnors och barns hälsa, Avdelningen för pediatrik,Institute for the Health of Women and Children, Dept of Paediatrics,University of Gothenburg
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- Abrahamsson, Katarina, 1957 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- Elsevier BV, 2005
- 2005
- Engelska.
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Ingår i: Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy. - : Elsevier BV. - 1386-1425. ; 61:4, s. 755-760
- Relaterad länk:
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http://dx.doi.org/10...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- This paper is aimed to show the possibility to determine individual organic compounds introduced into single living cells with surface-enhanced Raman spectroscopy (SERS). Surface enhancement was achieved with gold colloids that were allowed to diffuse into lymphocytes. An introduced analyte, rhodamine 6G, could be imaged together with for example nucleotides and amino acids of the cell. Multivariate evaluation of surface-enhanced Raman images proved to be a powerful tool for the separation of spectral information of various intracellular components. The principal component analysis (PCA) enabled identification of spectra containing different chemical information and separation of the spectral contribution of rhodamine 6G from the complex cellular matrix.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- surface-enhanced Raman scattering
- SERS
- chemical imaging
- single cells
- lymphocyte
- rhodamine 6G
- principal components analysis
- PCA
- multivariate analysis
- single cells
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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