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  • Kumar, SarojLinnéuniversitetet,Institutionen för kemi och biomedicin (KOB) (författare)

Magnetic capture from blood rescues molecular motor function in diagnostic nanodevices

  • Artikel/kapitelEngelska2013

Förlag, utgivningsår, omfång ...

  • 2013-05-03
  • BioMed Central (BMC),2013
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:oru-99361
  • https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-99361URI
  • https://doi.org/10.1186/1477-3155-11-14DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-27565URI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:gih:diva-6442URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Funding agency:Faculty of Natural Sciences and Engineering at Linnaeus University
  • Background: Introduction of effective point-of-care devices for use in medical diagnostics is part of strategies to combat accelerating health-care costs. Molecular motor driven nanodevices have unique potentials in this regard due to unprecedented level of miniaturization and independence of external pumps. However motor function has been found to be inhibited by body fluids.Results: We report here that a unique procedure, combining separation steps that rely on antibody-antigen interactions, magnetic forces applied to magnetic nanoparticles (MPs) and the specificity of the actomyosin bond, can circumvent the deleterious effects of body fluids (e.g. blood serum). The procedure encompasses the following steps: (i) capture of analyte molecules from serum by MP-antibody conjugates, (ii) pelleting of MP-antibody-analyte complexes, using a magnetic field, followed by exchange of serum for optimized biological buffer, (iii) mixing of MP-antibody-analyte complexes with actin filaments conjugated with same polyclonal antibodies as the magnetic nanoparticles. This causes complex formation: MP-antibody-analyte-antibody-actin, and magnetic separation is used to enrich the complexes. Finally (iv) the complexes are introduced into a nanodevice for specific binding via actin filaments to surface adsorbed molecular motors (heavy meromyosin). The number of actin filaments bound to the motors in the latter step was significantly increased above the control value if protein analyte (50-60 nM) was present in serum (in step i) suggesting appreciable formation and enrichment of the MP-antibody-analyte-antibody-actin complexes. Furthermore, addition of ATP demonstrated maintained heavy meromyosin driven propulsion of actin filaments showing that the serum induced inhibition was alleviated. Detailed analysis of the procedure i-iv, using fluorescence microscopy and spectroscopy identified main targets for future optimization.Conclusion: The results demonstrate a promising approach for capturing analytes from serum for subsequent motor driven separation/detection. Indeed, the observed increase in actin filament number, in itself, signals the presence of analyte at clinically relevant nM concentration without the need for further motor driven concentration. Our analysis suggests that exchange of polyclonal for monoclonal antibodies would be a critical improvement, opening for a first clinically useful molecular motor driven lab-on-a-chip device.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • ten Siethoff, LasseLinnéuniversitetet,Institutionen för kemi och biomedicin (KOB),Linneuniversitetet(Swepub:gih)lasse.tensiethoff (författare)
  • Persson, Malin,1983-Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB)(Swepub:lnu)fpema (författare)
  • Albet-Torres, NuriaLinnéuniversitetet,Institutionen för kemi och biomedicin (KOB)(Swepub:lnu)ealnu (författare)
  • Månsson, AlfLinnéuniversitetet,Institutionen för kemi och biomedicin (KOB)(Swepub:lnu)nmaal (författare)
  • LinnéuniversitetetInstitutionen för kemi och biomedicin (KOB) (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Journal of Nanobiotechnology: BioMed Central (BMC)111477-3155

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