SwePub
Sök i LIBRIS databas

  Utökad sökning

WFRF:(Alvarez Ruiz Jesus)
 

Sökning: WFRF:(Alvarez Ruiz Jesus) > iTRAQ-Based Proteom...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00005544naa a2200505 4500
001oai:DiVA.org:uu-418964
003SwePub
008200908s2020 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4189642 URI
0247 a https://doi.org/10.3389/fmicb.2020.010842 DOI
040 a (SwePub)uu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Alejandro Zamora-Briseno, Jesusu Inst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Lab Inmunol & Biol Mol,Dept Recursos Mar, Merida, Yucatan, Mexico.4 aut
2451 0a iTRAQ-Based Proteomic Profile Analysis of the Hepatopancreas of Caribbean Spiny Lobsters Infected WithPanulirus argusVirus 1 :b Metabolic and Physiological Implications
264 c 2020-05-29
264 1b Frontiers Media SA,c 2020
338 a electronic2 rdacarrier
520 a The Caribbean spiny lobsterPanulirus argus(Latreille, 1084) sustains economically valuable fisheries throughout the wider Caribbean region. This species is currently affected by the pathogenic virusPanulirus argusVirus 1 (PaV1) that causes a systemic and chronic-degenerative infection in juvenile spiny lobstersP. argus. To date, there is no available information regarding the host alterations induced by this pathogen at the molecular level. In the present study, comparative proteomic analyses of the changes in the hepatopancreas between infected and non-infected juvenile lobsters were analyzed by isobaric tags for relative and absolute quantitation (iTRAQ) coupled to synchronous precursor selection (SPS)-based MS3. We identified a total of 636 proteins, being 68 down-regulated and 71 up-regulated proteins. Among the down-regulated proteins, we identified several enzymes involved in the metabolism of hormones and lipids, digestive proteases and glycosidases, while proteins associated with the histone core, protein synthesis, immune response and RNA regulation were up-regulated. Several misregulated enzymes involved in the regulation of neuromodulators were also identified. RT-qPCR assays were used to validate the expression of transcripts encoding for selected differential proteins that were in concordance to proteomic data, as well as the tendency observed in the enzymatic activities of trypsin, chymotrypsin, and glycosidase. In a similar way, we observed glycogen reduction in muscle, and an increase in plasma acylglycerides and glucose, which may be explained by proteomic data. This study provides the first insight into the molecular changes in the hepatopancreas of Caribbean spiny lobsters associated to PaV1 infection. Data provided herein would help to clarify the origin of the molecular misregulations observed at macroscopic level in this host-pathogen interaction.
650 7a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
653 a PaV1
653 a hepatopancreas
653 a viral infection
653 a proteomics
653 a Panulirus argus
7001 a Ruiz-May, Elielu Cluster Cientif & Tecnol BioMimic, Inst Ecol, Red Estudios Mol Avanzados, Xalapa, Veracruz, Mexico.4 aut
7001 a Miguel Elizalde-Contreras, Joseu Cluster Cientif & Tecnol BioMimic, Inst Ecol, Red Estudios Mol Avanzados, Xalapa, Veracruz, Mexico.4 aut
7001 a Margarita Hernandez-Velazquez, Ioreniu Inst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Lab Inmunol & Biol Mol,Dept Recursos Mar, Merida, Yucatan, Mexico.4 aut
7001 a Hernandez-Perez, Ariadneu Uppsala universitet,Jämförande fysiologi4 aut0 (Swepub:uu)arihe244
7001 a Guadalupe Fuentes-Garcia, Anau Inst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Lab Inmunol & Biol Mol,Dept Recursos Mar, Merida, Yucatan, Mexico.4 aut
7001 a Herrera-Salvatierra, Nancyu Inst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Lab Inmunol & Biol Mol,Dept Recursos Mar, Merida, Yucatan, Mexico.4 aut
7001 a Briones-Fourzan, Patriciau Univ Nacl Autonoma Mexico, Isnt Ciencias Mar & Limnol, Unidad Acad Sistemas Arrecifales, Puerto Morelos, Mexico.4 aut
7001 a Pascual-Jimenez, Cristinau Univ Nacl Autonoma Mexico, Unidad Multidisciplinaria Docencia & Invest, Fac Ciencias, Sisal, Mexico.4 aut
7001 a Lozano-Alvarez, Enriqueu Univ Nacl Autonoma Mexico, Isnt Ciencias Mar & Limnol, Unidad Acad Sistemas Arrecifales, Puerto Morelos, Mexico.4 aut
7001 a Rodriguez-Canul, Rossannau Inst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Lab Inmunol & Biol Mol,Dept Recursos Mar, Merida, Yucatan, Mexico.4 aut
7102 a Inst Politecn Nacl, Unidad Merida, Ctr Invest & Estudios Avanzados, Lab Inmunol & Biol Mol,Dept Recursos Mar, Merida, Yucatan, Mexico.b Cluster Cientif & Tecnol BioMimic, Inst Ecol, Red Estudios Mol Avanzados, Xalapa, Veracruz, Mexico.4 org
7730 t Frontiers in Microbiologyd : Frontiers Media SAg 11q 11x 1664-302X
8564 u https://doi.org/10.3389/fmicb.2020.01084y Fulltext
8564 u https://uu.diva-portal.org/smash/get/diva2:1465004/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
8564 u https://www.frontiersin.org/articles/10.3389/fmicb.2020.01084/pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-418964
8564 8u https://doi.org/10.3389/fmicb.2020.01084

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy