SwePub
Sök i LIBRIS databas

  Extended search

WFRF:(Andersen M. I.)
 

Search: WFRF:(Andersen M. I.) > (2010-2014) > Interlaboratory Stu...

  • Leymarie, N. (author)

Interlaboratory Study on Differential Analysis of Protein Glycosylation by Mass Spectrometry: The ABRF Glycoprotein Research Multi-Institutional Study 2012

  • Article/chapterEnglish2013

Publisher, publication year, extent ...

  • 2013

Numbers

  • LIBRIS-ID:oai:gup.ub.gu.se/194786
  • https://gup.ub.gu.se/publication/194786URI
  • https://doi.org/10.1074/mcp.M113.030643DOI

Supplementary language notes

  • Language:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • One of the principal goals of glycoprotein research is to correlate glycan structure and function. Such correlation is necessary in order for one to understand the mechanisms whereby glycoprotein structure elaborates the functions of myriad proteins. The accurate comparison of glycoforms and quantification of glycosites are essential steps in this direction. Mass spectrometry has emerged as a powerful analytical technique in the field of glycoprotein characterization. Its sensitivity, high dynamic range, and mass accuracy provide both quantitative and sequence/structural information. As part of the 2012 ABRF Glycoprotein Research Group study, we explored the use of mass spectrometry and ancillary methodologies to characterize the glycoforms of two sources of human prostate specific antigen (PSA). PSA is used as a tumor marker for prostate cancer, with increasing blood levels used to distinguish between normal and cancer states. The glycans on PSA are believed to be biantennary N-linked, and it has been observed that prostate cancer tissues and cell lines contain more antennae than their benign counterparts. Thus, the ability to quantify differences in glycosylation associated with cancer has the potential to positively impact the use of PSA as a biomarker. We studied standard peptide-based proteomics/glycomics methodologies, including LC-MS/MS for peptide/glycopeptide sequencing and label-free approaches for differential quantification. We performed an interlaboratory study to determine the ability of different laboratories to correctly characterize the differences between glycoforms from two different sources using mass spectrometry methods. We used clustering analysis and ancillary statistical data treatment on the data sets submitted by participating laboratories to obtain a consensus of the glycoforms and abundances. The results demonstrate the relative strengths and weaknesses of top-down glycoproteomics, bottom-up glycoproteomics, and glycomics methods. T6G 2G2, Canada. [Cipollo, John F.; An, Yanming] US FDA, Ctr Biol Evaluat & Res, Bethesda, MD 20993 USA. [Desaire, Heather; Go, Eden P.] Univ Kansas, Lawrence, KS 66045 USA. [Goldman, Radoslav; Pompach, Petr; Sanda, Miloslav] Georgetown Univ, Dept Oncol, Washington, DC [Halim, Adnan; Larson, Goran; Nilsson, Jonas] Univ Gothenburg, Sahlgrenska Acad, Dept Clin Chem & [Hensbergen, Paul J.; Wuhrer, Manfred] Leiden Univ, Med Ctr, Biomol Mass Spectrometry Unit, NL- [Jabs, Wolfgang; Marx, Kristina; Resemann, Anja; Schweiger-Hufnagel, Ulrike; Suckau, Detlev] Bruker [Ly, Mellisa; Staples, Gregory O.] Agilent Technol, Agilent Labs, Santa Clara, CA 95051 USA. [Mechref, Yehia; Song, Ehwang] Texas Tech Univ, Dept Chem & Biochem, Lubbock, TX 79409 USA. [Nyalwidhe, Julius O.; Watson, Megan] Eastern Virginia Med Sch, Leroy T Canoles Jr Canc Res Ctr, Dept [Packer, Nicolle H.; Thaysen-Andersen, Morten] Macquarie Univ, Dept Chem & Biomol Sci, Biomol [Sihlbom, Carina] Gothenburg Univ, Prote Core Facil, Gothenburg, Sweden. [Tang, Haixu] Indiana Univ, Sch Informat, Bloomington, IN 47405 USA. [Valmuv, Leena] Finnish Red Cross Blood Serv, Helsinki 00310, Finland. [Wada, Yoshinao] Osaka Med Ctr Maternal & Child Hlth, Res Inst, Izumi Ku, Osaka 5941101, Japan.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Griffin, P. J. (author)
  • Jonscher, K. (author)
  • Kolarich, D. (author)
  • Orlando, R. (author)
  • McComb, M. (author)
  • Zaia, J. (author)
  • Aguilan, J. (author)
  • Alley, W. R. (author)
  • Altmann, F. (author)
  • Ball, L. E. (author)
  • Basumallick, L. (author)
  • Bazemore-Walker, C. R. (author)
  • Behnken, H. (author)
  • Blank, M. A. (author)
  • Brown, K. J. (author)
  • Bunz, S. C. (author)
  • Cairo, C. W. (author)
  • Cipollo, J. F. (author)
  • Daneshfar, R. (author)
  • Desaire, H. (author)
  • Drake, R. R. (author)
  • Go, E. P. (author)
  • Goldman, R. (author)
  • Gruber, C. (author)
  • Halim, AdnanGothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine(Swepub:gu)xhalad (author)
  • Hathout, Y. (author)
  • Hensbergen, P. J. (author)
  • Horn, D. M. (author)
  • Hurum, D. (author)
  • Jabs, W. (author)
  • Larson, Göran,1953Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine(Swepub:gu)xlarsg (author)
  • Ly, M. (author)
  • Mann, B. F. (author)
  • Marx, K. (author)
  • Mechref, Y. (author)
  • Meyer, B. (author)
  • Moginger, U. (author)
  • Neususs, C. (author)
  • Nilsson, Jonas,1970Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine(Swepub:gu)xnjoni (author)
  • Novotny, M. V. (author)
  • Nyalwidhe, J. O. (author)
  • Packer, N. H. (author)
  • Pompach, P. (author)
  • Reiz, B. (author)
  • Resemann, A. (author)
  • Rohrer, J. S. (author)
  • Ruthenbeck, A. (author)
  • Sanda, M. (author)
  • Schulz, J. M. (author)
  • Schweiger-Hufnagel, U. (author)
  • Sihlbom, Carina,1973Gothenburg University,Göteborgs universitet,Core Facilities, Proteomics,Core Facilities, Proteomics(Swepub:gu)xsihca (author)
  • Song, E. (author)
  • Staples, G. O. (author)
  • Suckau, D. (author)
  • Tang, H. X. (author)
  • Thaysen-Andersen, M. (author)
  • Viner, R. I. (author)
  • An, Y. M. (author)
  • Valmuv, L. (author)
  • Wada, Y. (author)
  • Watson, M. (author)
  • Windwarder, M. (author)
  • Whittal, R. (author)
  • Wuhrer, M. (author)
  • Zhu, Y. Y. (author)
  • Zou, C. X. (author)
  • Göteborgs universitetInstitutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin (creator_code:org_t)

Related titles

  • In:Molecular & Cellular Proteomics12:10, s. 2935-29511535-9476

Internet link

Find in a library

To the university's database

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view