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Extraction of Antio...
Extraction of Antioxidants from Spruce (Picea abies) Bark using Eco-Friendly Solvents
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- Co, Michelle, 1975- (författare)
- Uppsala universitet,Analytisk kemi,Dept Phys & Analyt Chem, Uppsala Univ, POB 599, SE-75124 Uppsala
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- Fagerlund, Amelie (författare)
- Mittuniversitetet,Uppsala universitet,Institutionen för biokemi och organisk kemi,Institutionen för tillämpad naturvetenskap och design (-2013)
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- Engman, Lars (författare)
- Uppsala universitet,Institutionen för kemi - BMC,Dept Biochem & Organ Chem, Uppsala Univ, SE-75123 Uppsala
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- Sunnerheim, Kerstin (författare)
- Mittuniversitetet,Institutionen för tillämpad naturvetenskap och design (-2013),Department of Natural Sciences, Engineering and Mathematics, Mid Sweden University
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- Sjöberg, Per J. R (författare)
- Uppsala universitet,Analytisk kemi,Dept Phys & Analyt Chem, Uppsala Univ, POB 599, SE-75124 Uppsala
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- Turner, Charlotta (författare)
- Lund University,Lunds universitet,Centrum för analys och syntes,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Centre for Analysis and Synthesis,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Dept Phys & Analyt Chem, Uppsala Univ, POB 599, SE-75124 Uppsala,Dept of Organic Chemistry, Lund University
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(creator_code:org_t)
- 2011-03-24
- 2012
- Engelska.
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Ingår i: Phytochemical Analysis. - : Wiley-Blackwell. - 0958-0344 .- 1099-1565. ; 23:1, s. 1-11
- Relaterad länk:
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http://dx.doi.org/10...
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http://onlinelibrary...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- Introduction-Antioxidants are known to avert oxidation processes and they are found in trees and other plant materials. Tree bark is a major waste product from paper pulp industries; hence it is worthwhile to develop an extraction technique to extract the antioxidants.Objective- To develop a fast and environmentally sustainable extraction technique for the extraction of antioxidants from bark of spruce (Picea abies) and also to identify the extracted antioxidants that are abundant in spruce bark.Methodology- A screening experiment that involved three different techniques, was conducted to determine the best technique to extract antioxidants.The antioxidant capacity of the extracts was determined with DPPH (2,2-diphenyl-2’-picrylhydrazyl) assay. Pressurised fluid extraction (PFE) turned out to be the best technique and a response surface design was therefore utilised to optimise PFE. Furthermore, NMR and HPLC-DAD-MS/MS were applied to identify the extracted antioxidants.Results- PFE using water and ethanol as solvent at 160 and 180°C, respectively, gave extracts of the highest antioxidant capacity. Stilbene glucosides such as isorhapontin, piceid and astringin were identified in the extracts.Conclusion-The study has shown that PFE is a fast and environmentally sustainable technique, using water and ethanol as solvent for the extraction of antioxidants from spruce bark.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Nyckelord
- Accelerated solvent extraction
- antioxidant
- DPPH
- ethanol
- Picea abies
- pressurised fluid extraction
- supercritical fluid extraction
- water
- Chemistry
- Kemi
- Analytical Chemistry
- Analytisk kemi
- accelerated solvent extraction
- antioxidant
- DPPH
- ethanol
- Picea abies
- pressurised fluid extraction
- supercritical fluid extraction
- water
- stilbene glucosides
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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