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Sökning: WFRF:(Deng Qiaolin) > Ovarian ERβ cistrom...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004609naa a2200505 4500
001oai:DiVA.org:kth-341540
003SwePub
008231222s2023 | |||||||||||000 ||eng|
009oai:prod.swepub.kib.ki.se:154395841
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3415402 URI
0247 a https://doi.org/10.1186/s12915-023-01773-12 DOI
0247 a http://kipublications.ki.se/Default.aspx?queryparsed=id:1543958412 URI
040 a (SwePub)kthd (SwePub)ki
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Birgersson, Madeleineu KTH,Science for Life Laboratory, SciLifeLab,Proteinvetenskap,Karolinska Inst, Dept Biosci & Nutr, S-14183 Huddinge, Sweden.4 aut0 (Swepub:kth)u1cwmzu4
2451 0a Ovarian ERβ cistrome and transcriptome reveal chromatin interaction with LRH-1
264 1b Springer Nature,c 2023
338 a print2 rdacarrier
500 a QC 20231222
520 a Background: Estrogen receptor beta (ERβ, Esr2) plays a pivotal role in folliculogenesis and ovulation, yet its exact mechanism of action is mainly uncharacterized.Results: We here performed ERβ ChIP-sequencing of mouse ovaries followed by complementary RNA-sequencing of wild-type and ERβ knockout ovaries. By integrating the ERβ cistrome and transcriptome, we identified its direct target genes and enriched biological functions in the ovary. This demonstrated its strong impact on genes regulating organism development, cell migration, lipid metabolism, response to hypoxia, and response to estrogen. Cell-type deconvolution analysis of the bulk RNA-seq data revealed a decrease in luteal cells and an increased proportion of theca cells and a specific type of cumulus cells upon ERβ loss. Moreover, we identified a significant overlap with the gene regulatory network of liver receptor homolog 1 (LRH-1, Nr5a2) and showed that ERβ and LRH-1 extensively bound to the same chromatin locations in granulosa cells. Using ChIP-reChIP, we corroborated simultaneous ERβ and LRH-1 co-binding at the ERβ-repressed gene Greb1 but not at the ERβ-upregulated genes Cyp11a1 and Fkbp5. Transactivation assay experimentation further showed that ERβ and LRH-1 can inhibit their respective transcriptional activity at classical response elements.Conclusions: By characterizing the genome-wide endogenous ERβ chromatin binding, gene regulations, and extensive crosstalk between ERβ and LRH-1, along with experimental corroborations, our data offer genome-wide mechanistic underpinnings of ovarian physiology and fertility.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng
653 a ChIP-seq
653 a ER beta
653 a Esr2
653 a LRH-1
653 a Nr5a2
653 a Ovary
653 a RNA-seq
7001 a Indukuri, Rajithau KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1h2gbg8
7001 a Lindquist, Linnéau Karolinska Institutet,KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab,Karolinska Inst, Dept Biosci & Nutr, S-14183 Huddinge, Sweden.4 aut0 (Swepub:kth)u14f1ler
7001 a Stepanauskaite, Linau KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab,Karolinska Inst, Dept Biosci & Nutr, S-14183 Huddinge, Sweden.4 aut0 (Swepub:kth)u1xd7zej
7001 a Luo, Qingu Karolinska Institutet4 aut
7001 a Deng, Qiaolinu Karolinska Inst, Dept Physiol & Pharmacol, S-14183 Huddinge, Sweden.4 aut
7001 a Archer, Amenau KTH,Cellulär och klinisk proteomik,Science for Life Laboratory, SciLifeLab,Karolinska Inst, Dept Biosci & Nutr, S-14183 Huddinge, Sweden.4 aut0 (Swepub:kth)u131bapq
7001 a Williams, Cecilia,c Professor,d 1969-u Karolinska Institutet,KTH,Science for Life Laboratory, SciLifeLab,Cellulär och klinisk proteomik,Karolinska Inst, Dept Biosci & Nutr, S-14183 Huddinge, Sweden.4 aut0 (Swepub:kth)u1ygqmuy
7102 a KTHb Science for Life Laboratory, SciLifeLab4 org
7730 t BMC Biologyd : Springer Natureg 21:1q 21:1x 1741-7007
8564 u https://doi.org/10.1186/s12915-023-01773-1y Fulltext
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-341540
8564 8u https://doi.org/10.1186/s12915-023-01773-1
8564 8u http://kipublications.ki.se/Default.aspx?queryparsed=id:154395841

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