Identification of CD66a and CD66b as the major galectin-3 receptor candidates in human neutrophils.

• The mammalian lectin galectin-3 is a potent stimulus of human neutrophils, provided that the receptor(s) for the lectin has been mobilized to the cell surface before activation. We have recently shown that the receptors for galectin-3 are stored in intracellular mobilizable granules. Here we show supportive evidence for this in that DMSO-differentiated (neutrophil-like) HL-60 cells, which lack gelatinase and specific granules, are nonresponsive when exposed to galectin-3. Neutrophil granules were subsequently used for isolation of galectin-3 receptors by affinity chromatography. Proteins eluted from a galectin-3-Sepharose column by lactose were analyzed on SDS-polyacrylamide gels and showed two major bands of 100 and 160 kDa and a minor band of 120 kDa. By immunoblotting, these proteins were shown to correspond to CD66a (160 kDa), CD66b (100 kDa), and lysosome-associated membrane glycoprotein-1 and -2 (Lamp-1 and -2; 120 kDa). The unresponsive HL-60 cells lacked the CD66 Ags but contained the Lamps, implying that neutrophil CD66a and/or CD66b may be the functional galectin-3 receptors. This conclusion was supported by the subcellular localization of the CD66 proteins to the gelatinase and specific granules in resting neutrophils.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Immunology in the medical area (hsv//eng)

Nyckelord

Antigens

CD

biosynthesis

blood

metabolism

Antigens

Differentiation

biosynthesis

blood

metabolism

pharmacology

Carrier Proteins

blood

Cell Adhesion Molecules

Cell Differentiation

drug effects

Cytoplasmic Granules

metabolism

Dimethyl Sulfoxide

pharmacology

Galectin 3

Gelatinases

blood

HL-60 Cells

Humans

Lysosome-Associated Membrane Glycoproteins

Macrophage-1 Antigen

biosynthesis

Membrane Glycoproteins

biosynthesis

Neutrophil Activation

immunology

Neutrophils

immunology

metabolism

Receptors

Mitogen

biosynthesis

blood

metabolism

Subcellular Fractions

immunology

metabolism

Publikations- och innehållstyp

ref (ämneskategori)

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