No evidence for DNA N-6-methyladenine in mammals

Douvlataniotis, KarolosLinköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten,Region Östergötland, H.K.H. Kronprinsessan Victorias barn- och ungdomssjukhus (author)

Washington, DC, United States :American Association for the Advancement of Science (A A A S),2020
electronicrdacarrier

Funding Agencies|Swedish Research CouncilSwedish Research Council [2015-03495]; LiU-Cancer Network [2016-007]; Swedish Cancer SocietySwedish Cancer Society [CAN 2017/625]
N-6-methyladenine (6mdA) is a widespread DNA modification in bacteria. More recently, 6mdA has also been characterized in mammalian DNA. However, measurements of 6mdA abundance and profiles are often very dissimilar between studies, even when performed on DNA from identical mammalian cell types. Using comprehensive bioinformatics analyses of published data and novel experimental approaches, we reveal that efforts to assay 6mdA in mammals have been severely compromised by bacterial contamination, RNA contamination, technological limitations, and antibody nonspecificity. These complications render 6mdA an exceptionally problematic DNA modification to study and have resulted in erroneous detection of 6mdA in several mammalian systems. Together, our results strongly imply that the evidence published to date is not sufficient to support the presence of 6mdA in mammals.

Bensberg, MaikeLinköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten,Region Östergötland, H.K.H. Kronprinsessan Victorias barn- och ungdomssjukhus(Swepub:liu)maibe37 (author)
Lentini, AntonioLinköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten,Region Östergötland, H.K.H. Kronprinsessan Victorias barn- och ungdomssjukhus(Swepub:liu)antle32 (author)
Gylemo, BjörnLinköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten,Region Östergötland, H.K.H. Kronprinsessan Victorias barn- och ungdomssjukhus(Swepub:liu)bjogy93 (author)
Nestor, ColmLinköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten,Region Östergötland, H.K.H. Kronprinsessan Victorias barn- och ungdomssjukhus(Swepub:liu)colne37 (author)
Linköpings universitetAvdelningen för barns och kvinnors hälsa (creator_code:org_t)

In:Science AdvancesWashington, DC, United States : American Association for the Advancement of Science (A A A S)6:122375-2548

By the author/editor: Douvlataniotis, ...; Bensberg, Maike; Lentini, Antonio; Gylemo, Björn; Nestor, Colm

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

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