Characterization of Mast Cells from Healthy and Varicose Human Saphenous Vein

Mast cells (MCs) are distributed in tissues throughout the body and are highly involved in many physiological and pathophysiological processes. The potential and involvement of different MC phenotypes are still not well understood. MCs are present in blood vessel walls, but their specific phenotypic features are unknown. We aimed at characterizing MCs from human saphenous veins for localization, mediator content, and receptor expression. This was done in MCs from both healthy and varicose human saphenous veins (hSV and vSV, respectively). For both vSV and hSV, we found that vein MCs are mainly present in the tunica adventitia (99% MCs in adventitia) and that the population consists of both MCT and MCTC phenotypes (vSV: 55% MCT, hSV: 64% MCT). The vein MCs contained high levels of histamine (vSV: 27 pg/MC, hSV: 55 pg/MC) and tryptase (vSV: 98 pg/MC, hSV: 111 pg/MC), indicating a strong potential for regulatory effects on blood vessels. The receptor expression of FcɛRI, MRGPRX2, PTAFR, C3aR, and C5aR was found, even though the percentage of positive cells differed between vSV and hSV MCs. We conclude that vein MCs from the blood vessel wall have a high potential to affect the tissue around them.

Mogren, SofiaLund University,Lunds universitet,Respiratorisk cellbiologi,Forskargrupper vid Lunds universitet,Respiratory Cell Biology,Lund University Research Groups(Swepub:lu)so0187mo (författare)
Berlin, FridaLund University,Lunds universitet,Respiratorisk cellbiologi,Forskargrupper vid Lunds universitet,Respiratory Cell Biology,Lund University Research Groups(Swepub:lu)fr4827be (författare)
Andersson, CeciliaLund University,Lunds universitet,Respiratorisk cellbiologi,Forskargrupper vid Lunds universitet,Respiratory Cell Biology,Lund University Research Groups(Swepub:lu)ce4613an (författare)
Schmidt, SusanneCopenhagen University Hospital (författare)
Klitfod, LotteGentofte Hospital (författare)
Esteban, VanesaHospital Fundación Jiménez Díaz (författare)
Poulsen, Lars K.Copenhagen University Hospital (författare)
Jensen, Bettina M.Copenhagen University Hospital (författare)
Copenhagen University HospitalRespiratorisk cellbiologi (creator_code:org_t)

Av författaren/redakt...: Callesen, Katrin ...; Mogren, Sofia; Berlin, Frida; Andersson, Cecil ...; Schmidt, Susanne; Klitfod, Lotte; visa fler...; Esteban, Vanesa; Poulsen, Lars K.; Jensen, Bettina ...; visa färre...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Hematologi

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