Type 1 Diabetes Mellitus Donor Mesenchymal. Stromal Cells Exhibit Comparable Potency to Healthy Controls In Vitro

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Type 1 Diabetes Mellitus Donor Mesenchymal. Stromal Cells Exhibit Comparable Potency to Healthy Controls In Vitro

Davies, Lindsay C. (författare): Karolinska Institutet,Karolinska Institute, Sweden; Karolinska University Hospital, Sweden

Alm, Jessica J. (författare): Karolinska Institutet,Karolinska Institute, Sweden; Karolinska University Hospital, Sweden

Heldring, Nina (författare): Karolinska Institutet,Karolinska Institute, Sweden; Karolinska University Hospital, Sweden

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Moll, Guido (författare): Karolinska Inst, Ctr Hematol & Regenerat Med, Stockholm, Sweden.;Karolinska Inst, Dept Lab Med, Div Clin Immunol, Stockholm, Sweden.;Karolinska Inst, Dept Lab Med, Div Transfus Med, Stockholm, Sweden.;Karolinska Univ Hosp, Stockholm, Sweden.,Karolinska Institute, Sweden; Karolinska University Hospital, Sweden

Gavin, Caroline (författare): Karolinska Institutet,Karolinska Institute, Sweden; Karolinska University Hospital, Sweden

Batsis, Ioannis (författare): Karolinska Inst, Ctr Hematol & Regenerat Med, Stockholm, Sweden.,Karolinska Institute, Sweden

Qian, Hong (författare): Karolinska Institutet,Karolinska Institute, Sweden

Sigvardsson, Mikael (författare): Linköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten

Nilsson, Bo (författare): Uppsala universitet,Klinisk immunologi,Uppsala University, Sweden

Kyllonen, Lauri E. (författare): Helsinki Univ Hosp, Div Transplantat, Helsinki, Finland.,Helsinki University Hospital, Finland

Salmela, Kaija T. (författare): Helsinki Univ Hosp, Div Transplantat, Helsinki, Finland.,Helsinki University Hospital, Finland

Carlsson, Per-Ola (författare): Uppsala universitet,Institutionen för medicinsk cellbiologi,Institutionen för medicinska vetenskaper,Uppsala University, Sweden

Korsgren, Olle (författare): Uppsala universitet,Klinisk immunologi,Uppsala University, Sweden

Le Blanc, Katarina (författare): Karolinska Institutet,Karolinska Institute, Sweden; Karolinska University Hospital, Sweden

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(creator_code:org_t)

2016-07-13
2016
Engelska.
Ingår i: Stem Cells Translational Medicine. - : Oxford University Press (OUP). - 2157-6564 .- 2157-6580. ; 5:11, s. 1485-1495

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Tidskriftsartikel (refereegranskat)

Abstract Ämnesord

Stäng

Bone marrow mesenchymal stromal cells (BM-MSCs) have been characterized and used in many clinical studies based on their immunomodulatory and regenerative properties. We have recently reported the benefit of autologous MSC systemic therapy in the treatment of type 1 diabetes mellitus (T1D). Compared with allogeneic cells, use of autologous products reduces the risk of eliciting undesired complications in the recipient, including rejection, immunization, and transmission of viruses and prions; however, comparable potency of autologous cells is required for this treatment approach to remain feasible. To date, no analysis has been reported that phenotypically and functionally characterizes MSCs derived from newly diagnosed and late-stage T1D donors in vitro with respect to their suitability for systemic immunotherapy. In this study, we used gene array in combination with functional in vitro assays to address these questions. MSCs from T1D donors and healthy controls were expanded from BM aspirates. BM mononuclear cell counts and growth kinetics were comparable between the groups, with equivalent colony-forming unit-fibroblast capacity. Gene microarrays demonstrated differential gene expression between healthy and late-stage T1D donors in relation to cytokine secretion, immunomodulatory activity, and wound healing potential. Despite transcriptional differences, T1D MSCs did not demonstrate a significant difference from healthy controls in immunosuppressive activity, migratory capacity, or hemocompatibility. We conclude that despite differential gene expression, expanded MSCs from T1D donors are phenotypically and functionally similar to healthy control MSCs with regard to their immunomodulatory and migratory potential, indicating their suitability for use in autologous systemic therapy.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)

Nyckelord

Mesenchymal stem cells
Diabetes
Cellular therapy
Immunosuppression
Adult human bone marrow

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Av författaren/redakt...: Davies, Lindsay ...; Alm, Jessica J.; Heldring, Nina; Moll, Guido; Gavin, Caroline; Batsis, Ioannis; visa fler...; Qian, Hong; Sigvardsson, Mik ...; Nilsson, Bo; Kyllonen, Lauri ...; Salmela, Kaija T ...; Carlsson, Per-Ol ...; Korsgren, Olle; Le Blanc, Katari ...; visa färre...

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