Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties

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Fältnamn	Indikatorer	Metadata
000		04417naa a2200697 4500
001		oai:DiVA.org:su-118282
003		SwePub
008		150615s2015 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
009		oai:DiVA.org:umu-104379
009		oai:prod.swepub.kib.ki.se:131270848
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-1182822 URI
024	7	a https://doi.org/10.1016/j.nano.2015.01.0032 DOI
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1043792 URI
024	7	a http://kipublications.ki.se/Default.aspx?queryparsed=id:1312708482 URI
040		a (SwePub)sud (SwePub)umud (SwePub)ki
041		a engb eng
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Nordin, Joel Z.u Karolinska Institutet4 aut
245	1 0	a Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties
264	1	b Elsevier BV,c 2015
338		a print2 rdacarrier
520		a Extracellular vesicles (EVs) are natural nanoparticles that mediate intercellular transfer of RNA and proteins and are of great medical interest; serving as novel biomarkers and potential therapeutic agents. However, there is little consensus on the most appropriate method to isolate high-yield and high-purity EVs from various biological fluids. Here, we describe a systematic comparison between two protocols for EV purification: ultrafiltration with subsequent liquid chromatography (UF-LC) and differential ultracentrifugation (UC). A significantly higher EV yield resulted from UF-LC as compared to UC, without affecting vesicle protein composition. Importantly, we provide novel evidence that, in contrast to UC-purified EVs, the biophysical properties of UF-LC-purified EVs are preserved, leading toadifferent in vivo biodistribution, with less accumulation in lungs. Finally, we show that UF-LC is scalable and adaptable for EV isolation from complex media types such as stem cell media, which is of huge significance for future clinical applications involving EVs.
650	7	a NATURVETENSKAPx Kemi0 (SwePub)1042 hsv//swe
650	7	a NATURAL SCIENCESx Chemical Sciences0 (SwePub)1042 hsv//eng
650	7	a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe
650	7	a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Medicinsk bioteknologix Medicinsk bioteknologi0 (SwePub)304012 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Medical Biotechnologyx Medical Biotechnology0 (SwePub)304012 hsv//eng
653		a Biophysical properties
653		a extracellular vesicles
653		a size-exclusion liquid chromatography
653		a ultracentrifugation
653		a ultrafiltration
700	1	a Lee, Yiu Karolinska Institutet4 aut
700	1	a Vader, Pieter4 aut
700	1	a Mäger, Imre4 aut
700	1	a Johansson, Henrik J.u Karolinska Institutet4 aut
700	1	a Heusermann, Wolf4 aut
700	1	a Wiklander, Oscar P. B.u Karolinska Institutet4 aut
700	1	a Hällbrink, Mattiasu Stockholms universitet,Institutionen för neurokemi4 aut0 (Swepub:su)mhall
700	1	a Seow, Yiqi4 aut
700	1	a Bultema, Jarred J.4 aut
700	1	a Gilthorpe, Jonathanu Umeå universitet,Umeå centrum för molekylär medicin (UCMM)4 aut0 (Swepub:umu)jogi0002
700	1	a Davies, Tim4 aut
700	1	a Fairchild, Paul J.4 aut
700	1	a Gabrielsson, Susanneu Karolinska Institutet4 aut
700	1	a Meisner-Kober, Nicole C.4 aut
700	1	a Lehtio, Janneu Karolinska Institutet4 aut
700	1	a Smith, C. I. Edvardu Karolinska Institutet4 aut
700	1	a Wood, Matthew J. A.4 aut
700	1	a Andaloussi, Samir E. L.u Karolinska Institutet4 aut
710	2	a Karolinska Institutetb Institutionen för neurokemi4 org
773	0	t Nanomedicined : Elsevier BVg 11:4, s. 879-883q 11:4<879-883x 1549-9634x 1549-9642
856	4	u https://doi.org/10.1016/j.nano.2015.01.003y Fulltext
856	4	u https://doi.org/10.1016/j.nano.2015.01.003
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-118282
856	4 8	u https://doi.org/10.1016/j.nano.2015.01.003
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-104379
856	4 8	u http://kipublications.ki.se/Default.aspx?queryparsed=id:131270848

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