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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00005468nam a2200337 4500
001oai:lup.lub.lu.se:8e68a427-b4b2-4abd-91dc-6f2d652d6532
003SwePub
008220321s2022 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/8e68a427-b4b2-4abd-91dc-6f2d652d65322 URI
0247 a https://doi.org/10.1101/2022.03.15.4844112 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a ovr2 swepub-publicationtype
072 7a vet2 swepub-contenttype
1001 a Dickson, Elnau Lund University,Lunds universitet,Biomarkörer vid hjärnsjukdomar,Forskargrupper vid Lunds universitet,Biomarkers in Brain Disease,Lund University Research Groups4 aut0 (Swepub:lu)el3025di
2451 0a Microarray profiling of hypothalamic gene expression changes in Huntington’s disease mouse models
264 1b Cold Spring Harbor Laboratory,c 2022
520 a Structural changes and neuropathology in the hypothalamus have been suggested to contribute to the non-motor manifestations of Huntington’s disease (HD), a neurodegenerative disorder caused by an expanded CAG repeat in the huntingtin (HTT) gene. In the present study, we investigated whether transcriptional changes would be part of hypothalamic pathology induced by the disease-causing huntingtin (HTT) protein. We performed microarray analysis using the Affymetrix platform on total hypothalamic RNA isolated from two HD mouse models and their littermate controls; BACHD mice with ubiquitous expression of full-length mutant HTT (mHTT) and wild-type mice with targeted hypothalamic overexpression of either wild-type HTT (wtHTT) or mHTT fragments. To analyze microarray datasets (34760 variables) and obtain functional implications of differential expression patterns, we used Linear Models for Microarray Data (limma) followed by Gene Set Enrichment Analysis (GSEA) using ClusterProfiler. Limma identified 735 and 721 significantly differentially expressed genes (adjusted p < 0.05) in hypothalamus of AAV datasets wtHTT vs control and mHTT vs control. In contrast, for BACHD datasets and the AAV mHTT vs. wtHTT dataset, none of the genes were differentially expressed (adjusted p-value > 0.05 for all probe IDs). In AAV groups, from the combined limma with GSEA using ClusterProfiler, we found both shared and unique gene sets and pathways for mice with wtHTT overexpression compared to mice with mHTT overexpression. mHTT caused widespread suppression of neuroendocrine networks, as evident by GSEA enrichment of GO-terms related to neurons and/or specific neuroendocrine populations. Using qRT-PCR, we confirmed that mHTT overexpression caused significant downregulation of key enzymes involved in neuropeptide synthesis, including histidine and dopa decarboxylases, compared to wtHTT overexpression. Multiple biosynthetic pathways such as sterol synthesis were among the top shared processes, where both unique and shared genes constituted leading-edge subsets. In conclusion, mice with targeted overexpression of HTT (wtHTT or mHTT) in the hypothalamus show dysregulation of pathways, of which there are subsets of shared pathways and pathways unique to either wtHTT or mHTT overexpression.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Neurovetenskaper0 (SwePub)301052 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Neurosciences0 (SwePub)301052 hsv//eng
7001 a Sai Dwijesha, Amoolyau Lund University,Lunds universitet,Translationell neuroendokrinologi,Forskargrupper vid Lunds universitet,Translational Neuroendocrinology,Lund University Research Groups4 aut0 (Swepub:lu)am6302sa
7001 a Andersson, Natalieu Lund University,Lunds universitet,Avdelningen för klinisk genetik,Institutionen för laboratoriemedicin,Medicinska fakulteten,Cancercellers evolution,Forskargrupper vid Lunds universitet,Division of Clinical Genetics,Department of Laboratory Medicine,Faculty of Medicine,Pathways of cancer cell evolution,Lund University Research Groups4 aut0 (Swepub:lu)na8883an
7001 a Hult Lundh, Sofiau Lund University,Lunds universitet,Translationell neuroendokrinologi,Forskargrupper vid Lunds universitet,Translational Neuroendocrinology,Lund University Research Groups,Novo Nordisk A/S4 aut0 (Swepub:lu)med-sfh
7001 a Björkqvist, Mariau Lund University,Lunds universitet,Biomarkörer vid hjärnsjukdomar,Forskargrupper vid Lunds universitet,Biomarkers in Brain Disease,Lund University Research Groups4 aut0 (Swepub:lu)farm-mbj
7001 a Petersén, Åsau Lund University,Lunds universitet,Translationell neuroendokrinologi,Forskargrupper vid Lunds universitet,Psykiatri, Lund,Sektion IV,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Translational Neuroendocrinology,Lund University Research Groups,Psychiatry (Lund),Section IV,Department of Clinical Sciences, Lund,Faculty of Medicine4 aut0 (Swepub:lu)mphy-apn
7001 a Soylu Kucharz, Ranau Lund University,Lunds universitet,Biomarkörer vid hjärnsjukdomar,Forskargrupper vid Lunds universitet,Biomarkers in Brain Disease,Lund University Research Groups4 aut0 (Swepub:lu)med-rso
7102 a Biomarkörer vid hjärnsjukdomarb Forskargrupper vid Lunds universitet4 org
8564 u http://dx.doi.org/10.1101/2022.03.15.484411x freey FULLTEXT
8564 8u https://lup.lub.lu.se/record/8e68a427-b4b2-4abd-91dc-6f2d652d6532
8564 8u https://doi.org/10.1101/2022.03.15.484411

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