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CD138 and CD31 Doub...
CD138 and CD31 Double-Positive Cells Comprise the Functional Antibody-Secreting Plasma Cell Compartment in Primate Bone Marrow
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- Martinez-Murillo, Paola (författare)
- Karolinska Institutet
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- Pramanik, Lotta (författare)
- Karolinska Institutet
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- Sundling, Christopher (författare)
- Karolinska Institutet
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- Hultenby, Kjell (författare)
- Karolinska Institutet
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- Wretenberg, Per, 1963- (författare)
- Department of Orthopedic Surgery, Karolinska University Hosptial, Stockholm, Sweden
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- Spångberg, Mats (författare)
- Medicine, Astrid Fagraeus Laboratory, Karolinska Institutet, Stockholm, Sweden
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- Karlsson Hedestam, Gunilla B. (författare)
- Karolinska Institutet
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(creator_code:org_t)
- 2016-06-27
- 2016
- Engelska.
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Ingår i: Frontiers in Immunology. - Lausanne, Switzerland : Frontiers Media S.A.. - 1664-3224. ; 7
- Relaterad länk:
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https://doi.org/10.3...
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https://www.frontier...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Plasma cells (PCs) are defined as terminally differentiated B cells that secrete large amounts of immunoglobulin (Ig). PCs that reside in the bone marrow (BM) are responsible for maintaining long-term antibody (Ab) responses after infection and vaccination, while PCs present in the blood are generally short-lived. In rhesus macaques, a species frequently used for the evaluation of human vaccines, B cells resemble those found in humans. However, a detailed characterization of BM-resident rhesus PC phenotype and function is lacking. Here, we examined Ig secretion of distinct rhesus CD138+ populations by B cell ELISpot analysis to couple phenotype with function. We demonstrate that the CD20low/-CD138+CD31+ BM population was highly enriched for antibody-secreting cells with IgG being the predominant isotype (60%), followed by IgA (33%) and IgM (7%). Transmission electron microscopy analysis confirmed PC enrichment in the CD20low/-CD138+CD31+ population with cells containing nuclei with "spokes of a wheel" chromatin structure and prominent rough endoplasmic reticulum. This panel also stained human BM PCs and allowed a clear distinction between BM PCs and short-lived peripheral PCs, providing an improved strategy to isolate PCs from rhesus BM for further analysis.
Ämnesord
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
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