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Comprehensive mappi...
Comprehensive mapping of neurotransmitter networks by MALDI-MS imaging
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- Shariatgorji, Mohammadreza (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Science for Life Laboratory, SciLifeLab
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- Nilsson, Anna (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Science for Life Laboratory, SciLifeLab
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- Fridjonsdottir, Elva (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Vallianatou, Theodosia (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Science for Life Laboratory, SciLifeLab
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- Källbäck, Patrik (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Katan, Luay (author)
- Uppsala universitet,Preparativ läkemedelskemi
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- Sävmarker, Jonas, 1979- (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Preparativ läkemedelskemi
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- Mantas, Ioannis (author)
- Karolinska Institutet
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- Zhang, Xiaoqun (author)
- Karolinska Institutet
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- Bezard, Erwan (author)
- Univ Bordeaux, Inst Malad Neurodegenerat, Bordeaux, France;CNRS, Inst Malad Neurodegenerat, Bordeaux, France
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- Svenningsson, Per (author)
- Karolinska Institutet
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- Odell, Luke R. (author)
- Uppsala universitet,Preparativ läkemedelskemi
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- Andrén, Per E. (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- 2019-09-23
- 2019
- English.
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In: Nature Methods. - : NATURE PUBLISHING GROUP. - 1548-7091 .- 1548-7105. ; 16:10, s. 1021-1028
- Related links:
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Abstract
Subject headings
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- We present a mass spectrometry imaging (MSI) approach for the comprehensive mapping of neurotransmitter networks in specific brain regions. Our fluoromethylpyridinium-based reactive matrices facilitate the covalent charge-tagging of molecules containing phenolic hydroxyl and/or primary or secondary amine groups, including dopaminergic and serotonergic neurotransmitters and their associated metabolites. These matrices improved the matrix-assisted laser desorption/ionization (MALDI)-MSI detection limit toward low-abundance neurotransmitters and facilitated the simultaneous imaging of neurotransmitters in fine structures of the brain at a lateral resolution of 10 mu m. We demonstrate strategies for the identification of unknown molecular species using the innate chemoselectivity of the reactive matrices and the unique isotopic pattern of a brominated reactive matrix. We illustrate the capabilities of the developed method on Parkinsonian brain samples from human post-mortem tissue and animal models. The direct imaging of neurotransmitter systems provides a method for exploring how various neurological diseases affect specific brain regions through neurotransmitter modulation.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Shariatgorji, Mo ...
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Nilsson, Anna
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Fridjonsdottir, ...
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Vallianatou, The ...
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Källbäck, Patrik
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Katan, Luay
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show more...
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Sävmarker, Jonas ...
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Mantas, Ioannis
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Zhang, Xiaoqun
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Bezard, Erwan
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Svenningsson, Pe ...
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Odell, Luke R.
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Andrén, Per E.
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Analytical Chemi ...
- Articles in the publication
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Nature Methods
- By the university
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Uppsala University
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Karolinska Institutet