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Broad Ultrastructur...
Broad Ultrastructural and Transcriptomic Changes Underlie the Multinucleated Giant Hemocyte Mediated Innate Immune Response against Parasitoids
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- Cinege, Gyöngyi (författare)
- Institute of Genetics, Innate Immunity Group, Immunology Unit, Biological Research Centre, Szeged, Hungary
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- Magyar, Lilla B. (författare)
- Institute of Genetics, Innate Immunity Group, Immunology Unit, Biological Research Centre, Szeged, Hungary; Doctoral School of Biology, University of Szeged, Szeged, Hungary
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- Kovács, Attila L. (författare)
- Department of Anatomy Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary
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- Lerner, Zita (författare)
- Institute of Genetics, Innate Immunity Group, Immunology Unit, Biological Research Centre, Szeged, Hungary; Doctoral School of Biology, University of Szeged, Szeged, Hungary
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- Juhász, Gábor (författare)
- Department of Anatomy Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary
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- Lukacsovich, David (författare)
- Laboratory of Neural Connectivity, Brain Research Institute, University of Zurich, Zurich, Switzerland
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- Winterer, Jochen (författare)
- Laboratory of Neural Connectivity, Brain Research Institute, University of Zurich, Zurich, Switzerland
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- Lukacsovich, Tamás (författare)
- Laboratory of Neural Connectivity, Brain Research Institute, University of Zurich, Zurich, Switzerland
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- Hegedus, Zoltán (författare)
- Laboratory of Bioinformatics, Biological Research Centre, Szeged, Hungary; Department of Biochemistry and Medical Chemistry, Medical School, University of Pécs, Pécs, Hungary
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- Kurucz, Éva (författare)
- Institute of Genetics, Innate Immunity Group, Immunology Unit, Biological Research Centre, Szeged, Hungary
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- Hultmark, Dan, 1949- (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Földy, Csaba (författare)
- Laboratory of Neural Connectivity, Brain Research Institute, University of Zurich, Zurich, Switzerland
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- Andó, István (författare)
- Institute of Genetics, Innate Immunity Group, Immunology Unit, Biological Research Centre, Szeged, Hungary
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(creator_code:org_t)
- 2021-12-03
- 2022
- Engelska.
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Ingår i: Journal of Innate Immunity. - : S. Karger. - 1662-811X .- 1662-8128. ; 14:4, s. 335-354
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Multinucleated giant hemocytes (MGHs) represent a novel type of blood cell in insects that participate in a highly efficient immune response against parasitoid wasps involving isolation and killing of the parasite. Previously, we showed that circulating MGHs have high motility and the interaction with the parasitoid rapidly triggers encapsulation. However, structural and molecular mechanisms behind these processes remained elusive. Here, we used detailed ultrastructural analysis and live cell imaging of MGHs to study encapsulation in Drosophila ananassae after parasitoid wasp infection. We found dynamic structural changes, mainly driven by the formation of diverse vesicular systems and newly developed complex intracytoplasmic membrane structures, and abundant generation of giant cell exosomes in MGHs. In addition, we used RNA sequencing to study the transcriptomic profile of MGHs and activated plasmatocytes 72 h after infection, as well as the uninduced blood cells. This revealed that differentiation of MGHs was accompanied by broad changes in gene expression. Consistent with the observed structural changes, transcripts related to vesicular function, cytoskeletal organization, and adhesion were enriched in MGHs. In addition, several orphan genes encoding for hemolysin-like proteins, pore-forming toxins of prokaryotic origin, were expressed at high level, which may be important for parasitoid elimination. Our results reveal coordinated molecular and structural changes in the course of MGH differentiation and parasitoid encapsulation, providing a mechanistic model for a powerful innate immune response.
Ämnesord
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
Nyckelord
- Drosophila
- Encapsulation
- Innate immunity
- Multinucleated giant hemocyte
- Transcriptome
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- art (ämneskategori)
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Cinege, Gyöngyi
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Magyar, Lilla B.
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Kovács, Attila L ...
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Lerner, Zita
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Juhász, Gábor
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Lukacsovich, Dav ...
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visa fler...
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Winterer, Jochen
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Lukacsovich, Tam ...
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Hegedus, Zoltán
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Kurucz, Éva
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Hultmark, Dan, 1 ...
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Földy, Csaba
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Andó, István
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Umeå universitet