An engineered Saccharomyces cerevisiae for cost-effective lignocellulosic bioethanol production: process performance and physiological insights

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Moreno, David,1986Chalmers tekniska högskola,Chalmers University of Technology (författare)

An engineered Saccharomyces cerevisiae for cost-effective lignocellulosic bioethanol production: process performance and physiological insights

Artikel/kapitelEngelska2015

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2015

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LIBRIS-ID:oai:research.chalmers.se:aa9c3fc6-1f3a-4fba-814e-645afc9aa6b2
https://research.chalmers.se/publication/223087URI

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Språk:engelska
Sammanfattning på:engelska

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The success in the commercialization of lignocellulosic bioethanol relies on the development of microorganisms with efficient hexose and pentose fermentation and tolerance towards inhibitory by-products (acetic acid, furan aldehydes and phenolics) generated during biomass processing. Traditionally, the yeast Saccharomyces cerevisiae is the preferred microorganism for industrial ethanol production. Many years of research and development have been conducted to develop S. cerevisiae strains suitable for fermenting lignocellulosic-based streams. S. cerevisiae is robust and ferment glucose efficiently, but it has been proved to be difficult to genetically modify for efficient xylose fermentation. In this work, a xylose-fermenting S. cerevisiae strain was subjected to evolutionary engineering, boosting its robustness and xylose fermentation capacity. The evolved strain was able to ferment a non-diluted enzymatic hydrolysate (representing 23% (w/w) dry matter of steam-exploded wheat straw), reaching ethanol titers higher than 5% (w/w) after 48 h. Within the first 24 h, glucose and xylose were co-consumed with rates of 3.1 and 0.7 g/L h, respectively, and converted to ethanol with yields corresponding to 93% of the theoretical. In addition, once glucose was depleted, xylose was consumed with a similar rate until reducing 70% of its initial concentration (36 h after inoculation). Besides investigating the fermentation parameters, the differences in gene expression levels and enzymatic activities of xylose-assimilating pathway were analyzed. These analyses will be the foundation for understanding the improved phenotype and the physiological mechanisms for efficient xylose fermentation, after which potential targets for subsequent metabolic engineering may be identified.

Ämnesord och genrebeteckningar

NATURVETENSKAP Biologi Evolutionsbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Evolutionary Biology hsv//eng
TEKNIK OCH TEKNOLOGIER Industriell bioteknik Bioenergi hsv//swe
ENGINEERING AND TECHNOLOGY Industrial Biotechnology Bioenergy hsv//eng
NATURVETENSKAP Biologi Mikrobiologi hsv//swe
NATURAL SCIENCES Biological Sciences Microbiology hsv//eng

Biuppslag (personer, institutioner, konferenser, titlar ...)

Tomas-Pejo, Elia,1980Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)elia (författare)
Geijer, Cecilia,1980Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)cecgei (författare)
Olsson, Lisbeth,1963Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)lisbetho (författare)
Chalmers tekniska högskola (creator_code:org_t)

Sammanhörande titlar

Ingår i:37th Symposium on Biotechnology for Fuels and Chemicals

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https://research.chalmers.se/publication/223087

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Av författaren/redakt...: Moreno, David, 1 ...; Tomas-Pejo, Elia ...; Geijer, Cecilia, ...; Olsson, Lisbeth, ...

Om ämnet

NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Evolutionsbiolog ...

TEKNIK OCH TEKNOLOGIER: TEKNIK OCH TEKNO ...; och Industriell biot ...; och Bioenergi

NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Mikrobiologi

Artiklar i publikationen: 37th Symposium o ...

Av lärosätet: Chalmers tekniska högskola

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