Functional pyruvate formate lyase pathway expressed with two different electron donors in Saccharomyces cerevisiae at aerobic growth

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Fältnamn	Indikatorer	Metadata
000		03267naa a2200433 4500
001		oai:research.chalmers.se:cf55de7f-6241-4c07-9597-d78f5b6331a8
003		SwePub
008		171008s2015 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://doi.org/10.1093/femsyr/fov0242 DOI
024	7	a https://research.chalmers.se/publication/2203602 URI
040		a (SwePub)cth
041		a engb eng
042		9 SwePub
072	7	a art2 swepub-publicationtype
072	7	a ref2 swepub-contenttype
100	1	a Zhang, Yiming,d 1986u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)yimingz
245	1 0	a Functional pyruvate formate lyase pathway expressed with two different electron donors in Saccharomyces cerevisiae at aerobic growth
264		c 2015-05-15
264	1	b Oxford University Press (OUP),c 2015
520		a Pyruvate formate lyase (PFL) is characterized as an enzyme functional at anaerobic conditions, since the radical in the enzyme's active form is sensitive to oxygen. In this study, PFL and its activating enzyme from Escherichia coli were expressed in a Saccharomyces cerevisiae strain lacking pyruvate decarboxylase and having a reduced glucose uptake rate due to a mutation in the transcriptional regulator Mth1, IMI076 (Pdc-MTH1-Delta T ura3-52). PFL was expressed with two different electron donors, reduced ferredoxin or reduced flavodoxin, respectively, and it was found that the coexpression of either of these electron donors had a positive effect on growth under aerobic conditions, indicating increased activity of PFL. The positive effect on growth was manifested as a higher final biomass concentration and a significant increase in transcription of formate dehydrogenases. Among the two electron donors reduced flavodoxin was found to be a better electron donor than reduced ferredoxin.
650	7	a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe
650	7	a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng
653		a ferredoxin/flavodoxin NADP(+) reductase
653		a ferredoxin
653		a metabolic engineering
653		a flavodoxin
653		a aerobic growth
653		a yeast
700	1	a Dai, Zongijie,d 1986u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)zongjie
700	1	a Krivoruchko, Anastasia,d 1984u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)anakri
700	1	a Chen, Yun,d 1978u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)yunc
700	1	a Siewers, Verena,d 1976u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)siewers
700	1	a Nielsen, Jens B,d 1962u Chalmers tekniska högskola,Chalmers University of Technology4 aut0 (Swepub:cth)nielsenj
710	2	a Chalmers tekniska högskola4 org
773	0	t FEMS Yeast Researchd : Oxford University Press (OUP)g 15:4q 15:4x 1567-1356x 1567-1364
856	4	u http://dx.doi.org/10.1093/femsyr/fov024y FULLTEXT
856	4	u https://academic.oup.com/femsyr/article-pdf/15/4/fov024/16742108/fov024.pdf
856	4 8	u https://doi.org/10.1093/femsyr/fov024
856	4 8	u https://research.chalmers.se/publication/220360

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Av författaren/redakt...: Zhang, Yiming, 1 ...; Dai, Zongijie, 1 ...; Krivoruchko, Ana ...; Chen, Yun, 1978; Siewers, Verena, ...; Nielsen, Jens B, ...

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