Dynamic range and reproducibility of hepatitis B virus (HBV) DNA detection and quantification by Cobas Taqman HBV, a real-time semiautomated assay.

• The Cobas Taqman assay for hepatitis B virus (HBV) DNA showed linear detection over 7 logs for genotypes A to D. The coefficient of variation was 1.2% at > or =1,000 IU/ml and 22.0% at 10 IU/ml. In 97 clinical samples, the log HBV DNA/ml differed by 0.11 between Cobas Amplicor and Cobas Taqman (r2 = 0.97).

Nyckelord

Automation

DNA

Viral

analysis

Genotype

Hepatitis B virus

genetics

Humans

Liver Transplantation

Polymerase Chain Reaction

methods

Reproducibility of Results

Viremia

virology

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