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Massive and rapid C...
Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR
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- Smyrlaki, I (författare)
- Karolinska Institutet
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Ekman, M (författare)
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- Lentini, A (författare)
- Karolinska Institutet
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visa fler...
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- de Sousa, NR (författare)
- Karolinska Institutet
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- Papanicolaou, N (författare)
- Karolinska Institutet
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Vondracek, M (författare)
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Aarum, J (författare)
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Safari, H (författare)
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Muradrasoli, S (författare)
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- Rothfuchs, AG (författare)
- Karolinska Institutet
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- Albert, J (författare)
- Karolinska Institutet
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- Hogberg, B (författare)
- Karolinska Institutet
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- Reinius, B (författare)
- Karolinska Institutet
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(creator_code:org_t)
- 2020-09-23
- 2020
- Engelska.
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Ingår i: Nature communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 11:1, s. 4812-
- Relaterad länk:
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https://www.nature.c...
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http://kipublication...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is commonly diagnosed by reverse transcription polymerase chain reaction (RT-PCR) to detect viral RNA in patient samples, but RNA extraction constitutes a major bottleneck in current testing. Methodological simplification could increase diagnostic availability and efficiency, benefitting patient care and infection control. Here, we describe methods circumventing RNA extraction in COVID-19 testing by performing RT-PCR directly on heat-inactivated or lysed samples. Our data, including benchmarking using 597 clinical patient samples and a standardised diagnostic system, demonstrate that direct RT-PCR is viable option to extraction-based tests. Using controlled amounts of active SARS-CoV-2, we confirm effectiveness of heat inactivation by plaque assay and evaluate various generic buffers as transport medium for direct RT-PCR. Significant savings in time and cost are achieved through RNA-extraction-free protocols that are directly compatible with established PCR-based testing pipelines. This could aid expansion of COVID-19 testing.
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Till lärosätets databas
- Av författaren/redakt...
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Smyrlaki, I
-
Ekman, M
-
Lentini, A
-
de Sousa, NR
-
Papanicolaou, N
-
Vondracek, M
-
visa fler...
-
Aarum, J
-
Safari, H
-
Muradrasoli, S
-
Rothfuchs, AG
-
Albert, J
-
Hogberg, B
-
Reinius, B
-
visa färre...
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Karolinska Institutet