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A universal SARS-Co...
A universal SARS-CoV DNA vaccine inducing highly cross-reactive neutralizing antibodies and T cells
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Appelberg, S. (author)
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- Ahlén, G. (author)
- Karolinska Institutet
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Yan, J. (author)
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- Nikouyan, N. (author)
- Karolinska Institutet
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Weber, S. (author)
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Larsson, O. (author)
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Höglund, U. (author)
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- Aleman, S. (author)
- Karolinska Institutet
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Weber, F. (author)
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Perlhamre, E. (author)
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Apro, J. (author)
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Gidlund, E. -K (author)
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Tuvesson, O. (author)
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Salati, S. (author)
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Cadossi, M. (author)
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- Tegel, Hanna (author)
- KTH,Proteinvetenskap
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- Hober, Sophia, Professor, 1965- (author)
- KTH,Proteinteknologi
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- Frelin, L. (author)
- Karolinska Institutet
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- Mirazimi, A. (author)
- Karolinska Institutet
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- Sällberg, M. (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2022-09-02
- 2022
- English.
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In: EMBO Molecular Medicine. - : EMBO. - 1757-4676 .- 1757-4684. ; 14:10
- Related links:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- New variants in the SARS-CoV-2 pandemic are more contagious (Alpha/Delta), evade neutralizing antibodies (Beta), or both (Omicron). This poses a challenge in vaccine development according to WHO. We designed a more universal SARS-CoV-2 DNA vaccine containing receptor-binding domain loops from the huCoV-19/WH01, the Alpha, and the Beta variants, combined with the membrane and nucleoproteins. The vaccine induced spike antibodies crossreactive between huCoV-19/WH01, Beta, and Delta spike proteins that neutralized huCoV-19/WH01, Beta, Delta, and Omicron virus in vitro. The vaccine primed nucleoprotein-specific T cells, unlike spike-specific T cells, recognized Bat-CoV sequences. The vaccine protected mice carrying the human ACE2 receptor against lethal infection with the SARS-CoV-2 Beta variant. Interestingly, priming of cross-reactive nucleoprotein-specific T cells alone was 60% protective, verifying observations from humans that T cells protect against lethal disease. This SARS-CoV vaccine induces a uniquely broad and functional immunity that adds to currently used vaccines.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- DNA vaccine
- in vivo electroporation
- preclinical development
- SARS-CoV-2
- universal SARS vaccine
- angiotensin converting enzyme 2
- membrane protein
- neutralizing antibody
- nucleoprotein
- SARS-CoV-2 vaccine
- virus spike protein
- animal cell
- animal experiment
- animal model
- animal tissue
- Article
- controlled study
- coronavirus disease 2019
- cross reaction
- electroporation
- female
- histopathology
- immunity
- in vitro study
- in vivo study
- Leporidae
- mouse
- New Zealand White (rabbit)
- nonhuman
- preclinical study
- receptor binding
- SARS coronavirus
- SARS-CoV-2 Alpha
- SARS-CoV-2 Beta
- SARS-CoV-2 Delta
- SARS-CoV-2 Omicron
- T lymphocyte
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Appelberg, S.
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Ahlén, G.
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Yan, J.
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Nikouyan, N.
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Weber, S.
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Larsson, O.
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show more...
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Höglund, U.
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Aleman, S.
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Weber, F.
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Perlhamre, E.
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Apro, J.
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Gidlund, E. -K
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Tuvesson, O.
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Salati, S.
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Cadossi, M.
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Tegel, Hanna
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Hober, Sophia, P ...
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Frelin, L.
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Mirazimi, A.
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Sällberg, M.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
- Articles in the publication
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EMBO Molecular M ...
- By the university
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Royal Institute of Technology
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Karolinska Institutet