WFRF:(Brandsdal Bjørn O.)
Sökning: WFRF:(Brandsdal Bjørn O.) > Structure and Mecha...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03987naa a2200409 4500 | |
| 001 | oai:DiVA.org:uu-476617 | |
| 003 | SwePub | |
| 008 | 220621s2022 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4766172 URI |
| 024 | 7 | a https://doi.org/10.1021/acs.biochem.2c000872 DOI |
| 040 | a (SwePub)uu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a van der Ent, Florianu Uppsala universitet,Beräkningsbiologi och bioinformatik4 aut0 (Swepub:uu)flova102 |
| 245 | 1 0 | a Structure and Mechanism of a Cold-Adapted Bacterial Lipase |
| 264 | c 2022-05-03 | |
| 264 | 1 | b American Chemical Society (ACS),c 2022 |
| 338 | a electronic2 rdacarrier | |
| 520 | a The structural origin of enzyme cold-adaptation has been the subject of considerable research efforts in recent years. Comparative studies of orthologous mesophilic-psychrophilic enzyme pairs found in nature are an obvious strategy for solving this problem, but they often suffer from relatively low sequence identity of the enzyme pairs. Small bacterial lipases adapted to distinctly different temperatures appear to provide an excellent model system for these types of studies, as they may show a very high degree of sequence conservation. Here, we report the first crystal structures of lipase A from the psychrophilic bacterium Bacillus pumilus, which confirm the high structural similarity to the mesophilic Bacillus subtilis enzyme, as indicated by their 81% sequence identity. We further employ extensive QM/MM calculations to delineate the catalytic reaction path and its energetics. The computational prediction of a rate-limiting deacylation step of the enzymatic ester hydrolysis reaction is verified by stopped-flow experiments, and steady-state kinetics confirms the psychrophilic nature of the B. pumilus enzyme. These results provide a useful benchmark for examining the structural basis of cold-adaptation and should now make it possible to disentangle the effects of the 34 mutations between the two enzymes on catalytic properties and thermal stability. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
| 700 | 1 | a Lund, Bjarte A.u Uppsala universitet,Institutionen för cell- och molekylärbiologi,Arctic Univ Norway, Univ Tromso, Dept Chem, Hylleraas Ctr Quantum Mol Sci, N-9037 Tromso, Norway.4 aut |
| 700 | 1 | a Svalberg, Linnu Uppsala universitet,Institutionen för cell- och molekylärbiologi4 aut0 (Swepub:uu)linsv807 |
| 700 | 1 | a Purg, Mihau Uppsala universitet,Institutionen för cell- och molekylärbiologi4 aut0 (Swepub:uu)mihpu392 |
| 700 | 1 | a Chukwu, Ghisleanu Uppsala universitet,Biokemi4 aut0 (Swepub:uu)ghich737 |
| 700 | 1 | a Widersten, Mikaelu Uppsala universitet,Biokemi4 aut0 (Swepub:uu)mikawide |
| 700 | 1 | a V. Isaksen, Geiru Arctic Univ Norway, Univ Tromso, Dept Chem, Hylleraas Ctr Quantum Mol Sci, N-9037 Tromso, Norway.4 aut |
| 700 | 1 | a Brandsdal, Bjørn O.u Arctic Univ Norway, Univ Tromso, Dept Chem, Hylleraas Ctr Quantum Mol Sci, N-9037 Tromso, Norway.4 aut |
| 700 | 1 | a Åqvist, Johanu Uppsala universitet,Beräkningsbiologi och bioinformatik,Arctic Univ Norway, Univ Tromso, Dept Chem, Hylleraas Ctr Quantum Mol Sci, N-9037 Tromso, Norway.4 aut0 (Swepub:uu)johanaq |
| 710 | 2 | a Uppsala universitetb Beräkningsbiologi och bioinformatik4 org |
| 773 | 0 | t Biochemistryd : American Chemical Society (ACS)g 61:10, s. 933-942q 61:10<933-942x 0006-2960x 1520-4995 |
| 856 | 4 | u https://doi.org/10.1021/acs.biochem.2c00087y Fulltext |
| 856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1673545/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-476617 |
| 856 | 4 8 | u https://doi.org/10.1021/acs.biochem.2c00087 |
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