Critical evaluation of the FANTOM3 non-coding RNA transcripts

We studied the genomic positions of 38,129 putative ncRNAs from the RIKEN dataset in relation to protein-coding genes. We found that the dataset has 41% sense, 6% antisense, 24% intronic and 29% intergenic transcripts. Interestingly, 17,678 (47%) of the FANTOM3 transcripts were found to potentially be internally primed from longer transcripts. The highest fraction of these transcripts was found among the intronic transcripts and as many as 77% or 6929 intronic transcripts were both internally primed and unspliced. We defined a filtered subset of 8535 transcripts that did not overlap with protein-coding genes, did not contain ORFs longer than 100 residues and were not internally primed. This dataset contains 53% of the FANTOM3 transcripts associated to known ncRNA in RNAdb and expands previous similar efforts with 6523 novel transcripts. This bioinformatic filtering of the FANTOM3 non-coding dataset has generated a lead dataset of transcripts without signs of being artefacts, providing a suitable dataset for investigation with hybridization-based techniques.

Mirza, Majd A IUppsala universitet,Funktionell farmakologi (författare)
Almén, Markus SällmanUppsala universitet,Funktionell farmakologi(Swepub:uu)marsa340 (författare)
Gloriam, David EUppsala universitet,Funktionell farmakologi (författare)
Fredriksson, RobertUppsala universitet,Funktionell farmakologi(Swepub:uu)rfr20930 (författare)
Schiöth, Helgi BUppsala universitet,Funktionell farmakologi(Swepub:uu)helgschi (författare)
Uppsala universitetFunktionell farmakologi (creator_code:org_t)

Av författaren/redakt...: Nordström, Karl ...; Mirza, Majd A I; Almén, Markus Sä ...; Gloriam, David E; Fredriksson, Rob ...; Schiöth, Helgi B

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