Sökning: WFRF:(Hamngren Blomqvist Charlotte 1984) >
Design and evaluati...
Design and evaluation of a microfluidic system for inhibition studies of yeast cell signaling
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- Hamngren Blomqvist, Charlotte, 1984 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Dinér, Peter (författare)
- Uppsala universitet,Organisk synteskemi,Göteborgs universitet,University of Gothenburg,Uppsala University,Department of Chemistry – BMC, Uppsala university
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- Grötli, Morten, 1966 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
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- Goksör, Mattias, 1975 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)
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- Adiels, Caroline B., 1976 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU)
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(creator_code:org_t)
- ISBN 9780819491756
- SPIE, 2012
- 2012
- Engelska.
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Ingår i: Proceedings of SPIE. - : SPIE. - 0277-786X .- 1996-756X. - 9780819491756 ; , s. 84582K-
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Abstract
Ämnesord
Stäng
- In cell signaling, different perturbations lead to different responses and using traditional biological techniques that result in averaged data may obscure important cell-to-cell variations. The aim of this study was to develop and evaluate a four-inlet microfluidic system that enables single-cell analysis by investigating the effect on Hog1 localization post a selective Hog1 inhibitor treatment during osmotic stress. Optical tweezers was used to position yeast cells in an array of desired size and density inside the microfluidic system. By changing the flow rates through the inlet channels, controlled and rapid introduction of two different perturbations over the cell array was enabled. The placement of the cells was determined by diffusion rates flow simulations. The system was evaluated by monitoring the subcellular localization of a fluorescently tagged kinase of the yeast "High Osmolarity Glycerol" (HOG) pathway, Hog1-GFP. By sequential treatment of the yeast cells with a selective Hog1 kinase inhibitor and sorbitol, the subcellular localization of Hog1-GFP was analysed on a single-cell level. The results showed impaired Hog1-GFP nuclear localization, providing evidence of a congenial design. The setup made it possible to remove and add an agent within 2 seconds, which is valuable for investigating the dynamic signal transduction pathways and cannot be done using traditional methods. We are confident that the features of the four-inlet microfluidic system will be a valuable tool and hence contribute significantly to unravel the mechanisms of the HOG pathway and similar dynamic signal transduction pathways.
Ämnesord
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Läkemedelskemi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medicinal Chemistry (hsv//eng)
Nyckelord
- Microfluidics
- Optical manipulation
- Signal transduction pathways
- Single-cell analysis
- Biological techniques
- Cell array
- Cell signaling
- Cell-to-cell variation
- Diffusion rate
- Dynamic signals
- HOG pathway
- Inlet channels
- Kinase inhibitors
- Micro fluidic system
- Nuclear localization
- Osmolarity
- Osmotic stress
- Sequential treatments
- Single-cell level
- Singlecell analysis
- Subcellular localizations
- Yeast cell
- Cells
- Cytology
- Fluidic devices
- Glycerol
- Micromanipulators
- Optical tweezers
- Signal transduction
- Yeast
- Molecular biology
- Microfluidics
- Optical manipulation
- Signal transduction pathways
- Single-cell analysis
Publikations- och innehållstyp
- ref (ämneskategori)
- kon (ämneskategori)
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