WFRF:(Järver Peter)
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Chemical synthesis and evaluation of a backbone-cyclized minimized 2-helix Z-domain
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- Järver, Peter (author)
- KTH,Molekylär Bioteknologi
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- Mikaelsson, Cecilia (author)
- KTH,Molekylär Bioteknologi
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- Karlstrom Eriksson, Amelie (author)
- KTH,Molekylär Bioteknologi
- (creator_code:org_t)
- 2011-02-24
- 2011
- English.
- In: Journal of Peptide Science. - : Wiley. - 1075-2617 .- 1099-1387. ; 17:6, s. 463-469
- Journal article (peer-reviewed)
Abstract
Subject headings
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- The Z-molecule is a small, engineered IgG-binding affinity protein derived from the immunoglobulin-binding domain B of Staphylococcus aureus protein A. The Z-domain consists of 58 amino acids forming a well-defined antiparallel three-helix structure. Two of the three helices are involved in ligand binding, whereas the third helix provides structural support to the three-helix bundle. The small size and the stable three-helix structure are two attractive properties comprised in the Z-domain, but a further reduction in size of the protein is valuable for several reasons. Reduction in size facilitates synthetic production of any protein-based molecule, which is beneficial from an economical viewpoint. In addition, a smaller protein is easier to manipulate through chemical modifications. By omitting the third stabilizing helix from the Z-domain and joining the N- and C-termini by a native peptide bond, the affinity protein obtains the advantageous properties of a smaller scaffold and in addition becomes resistant to exoproteases. We here demonstrate the synthesis and evaluation of a novel cyclic two-helix Z-domain. The molecule has retained affinity for its target protein, is resistant to heat treatment, and lacks both N- and C-termini. Copyright (C) 2011 European Peptide Society and John Wiley & Sons, Ltd.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- protein scaffold
- Z-domain
- two-helix protein
- SPPS
- IgG
- affinity purification
- Molecular biology
- Molekylärbiologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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