Search: WFRF:(Joshua Douglas)
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Distinct cervical t...
Distinct cervical tissue-adherent and luminal microbiome communities correlate with mucosal host gene expression and protein levels in Kenyan sex workers
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- Edfeldt, Gabriella (author)
- Karolinska Institutet
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- Kaldhusdal, Vilde (author)
- Karolinska Institutet
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- Czarnewski, Paulo (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Stockholm Univ, Dept Biochem & Biophys, Natl Bioinformat Infrastruct Sweden, SciLifeLab, Solna, Sweden.
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- Bradley, Frideborg (author)
- Karolinska Institutet
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- Bergström, Sofia (author)
- KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik
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- Lajoie, Julie (author)
- Univ Manitoba, Dept Med Microbiol & Infect Dis, Winnipeg, MB, Canada.
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- Xu, Jiawu (author)
- MIT & Harvard, Ragon Inst MGH, Cambridge, MA USA.
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- Månberg, Anna, 1985- (author)
- KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik
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- Kimani, Joshua (author)
- Univ Manitoba, Dept Med Microbiol & Infect Dis, Winnipeg, MB, Canada.;Univ Nairobi, Dept Med Microbiol, Nairobi, Kenya.;Partners Hlth & Dev Afr, Nairobi, Kenya.
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- Oyugi, Julius (author)
- Univ Manitoba, Dept Med Microbiol & Infect Dis, Winnipeg, MB, Canada.;Univ Nairobi, Dept Med Microbiol, Nairobi, Kenya.
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- Nilsson, Peter (author)
- KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik
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- Tjernlund, Annelie (author)
- Karolinska Univ Hosp, Karolinska Inst, Ctr Mol Med, Dept Med Solna,Div Infect Dis,Dept Infect Dis, J7 20, S-17176 Stockholm, Sweden.
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- Fowke, Keith R. (author)
- Univ Manitoba, Dept Med Microbiol & Infect Dis, Winnipeg, MB, Canada.;Univ Nairobi, Dept Med Microbiol, Nairobi, Kenya.;Partners Hlth & Dev Afr, Nairobi, Kenya.;Univ Manitoba, Dept Community Hlth Sci, Winnipeg, MB, Canada.
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- Kwon, Douglas S. (author)
- MIT & Harvard, Ragon Inst MGH, Cambridge, MA USA.
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- Broliden, Kristina (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2023-03-31
- 2023
- English.
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In: Microbiome. - : Springer Nature. - 2049-2618. ; 11:1
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
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- Background The majority of studies characterizing female genital tract microbiota have focused on luminal organisms, while the presence and impact of tissue-adherent ectocervical microbiota remain incompletely understood. Studies of luminal and tissue-associated bacteria in the gastrointestinal tract suggest that these communities may have distinct roles in health and disease. Here, we performed a multi-omics characterization of paired luminal and tissue samples collected from a cohort of Kenyan female sex workers.Results We identified a tissue-adherent bacterial microbiome, with a higher alpha diversity than the luminal microbiome, in which dominant genera overall included Gardnerella and Lactobacillus, followed by Prevotella, Atopobium, and Sneathia. About half of the L. iners-dominated luminal samples had a corresponding Gardnerella-dominated tissue microbiome. Broadly, the tissue-adherent microbiome was associated with fewer differentially expressed host genes than the luminal microbiome. Gene set enrichment analysis revealed that L. crispatus-dominated tissue-adherent communities were associated with protein translation and antimicrobial activity, whereas a highly diverse microbial community was associated with epithelial remodeling and pro-inflammatory pathways. Tissue-adherent communities dominated by L. iners and Gardnerella were associated with lower host transcriptional activity. Tissue-adherent microbiomes dominated by Lactobacillus and Gardnerella correlated with host protein profiles associated with epithelial barrier stability, although with a more pro-inflammatory profile for the Gardnerella-dominated microbiome group. Tissue samples with a highly diverse composition had a protein profile representing cell proliferation and pro-inflammatory activity.Conclusion We identified ectocervical tissue-adherent bacterial communities in all study participants of a female sex worker cohort. These communities were distinct from cervicovaginal luminal microbiota in a significant proportion of individuals. We further revealed that bacterial communities at both sites correlated with distinct host gene expression and protein levels. The tissue-adherent bacterial community could possibly act as a reservoir that seed the lumen with less optimal, non-Lactobacillus, bacteria.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Cervix
- Ectocervix
- Microbiota
- 16S rRNA gene
- Tissue
- Tissue-adherent
- Biofilm
- Luminal
- Transcriptomics
- Protein profiling
Publication and Content Type
- ref (subject category)
- art (subject category)
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Edfeldt, Gabriel ...
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Kaldhusdal, Vild ...
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Czarnewski, Paul ...
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Bradley, Fridebo ...
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Bergström, Sofia
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Lajoie, Julie
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Xu, Jiawu
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Månberg, Anna, 1 ...
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Kimani, Joshua
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Oyugi, Julius
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Nilsson, Peter
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Tjernlund, Annel ...
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Fowke, Keith R.
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Kwon, Douglas S.
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Broliden, Kristi ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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Microbiome
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Royal Institute of Technology
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Stockholm University
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Karolinska Institutet