Characterization of cross-species transcription and splicing from Penicillium to Saccharomyces cerevisiae

Nielsen, Jens B, 1962 (author): BioInnovation Institute (BII),Beijing University of Chemical Technology,Chalmers tekniska högskola,Chalmers University of Technology

(creator_code:org_t)

2021-08-13
2021
English.
In: Journal of Industrial Microbiology and Biotechnology. - : Oxford University Press (OUP). - 1367-5435 .- 1476-5535. ; 48:9-10

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Abstract Subject headings

Heterologous expression of eukaryotic gene clusters in yeast has been widely used for producing high-value chemicals and bioactive secondary metabolites. However, eukaryotic transcription cis-elements are still undercharacterized, and the cross-species expression mechanism remains poorly understood. Here we used the whole expression unit (including original promoter, terminator, and open reading frame with introns) of orotidine 5'-monophosphate decarboxylases from 14 Penicillium species as a showcase, and analyzed their cross-species expression in Saccharomyces cerevisiae. We found that pyrG promoters from the Penicillium species could drive URA3 expression in yeast, and that inefficient cross-species splicing of Penicillium introns might result in weak cross-species expression. Thus, this study demonstrates cross-species expression from Penicillium to yeast, and sheds light on the opportunities and challenges of cross-species expression of fungi expression units and gene clusters in yeast without refactoring for novel natural product discovery.

TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
NATURVETENSKAP -- Biologi -- Bioinformatik och systembiologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Bioinformatics and Systems Biology (hsv//eng)
NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)

By the author/editor: Lin, Zhenquan; Xu, Kang; Cai, Guang; Liu, Yangqingxue; Li, Yi; Zhang, Zhihao; show more...; Nielsen, Jens B, ...; Shi, Shuobo, 198 ...; Liu, Zihe, 1984; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Bioinformatics a ...

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