Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive T cell responses: position statement of the T-Cell Workshop Committee of the Immunology of Diabetes Society.

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Fältnamn	Indikatorer	Metadata
000		03069naa a2200385 4500
001		oai:lup.lub.lu.se:8aae796c-ff1d-405d-87e5-81afa0a421b0
003		SwePub
008		160404s2011 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://lup.lub.lu.se/record/17112542 URI
024	7	a https://doi.org/10.1111/j.1365-2249.2010.04272.x2 DOI
040		a (SwePub)lu
041		a engb eng
042		9 SwePub
072	7	a art2 swepub-publicationtype
072	7	a ref2 swepub-contenttype
100	1	a Mallone, R4 aut
245	1 0	a Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive T cell responses: position statement of the T-Cell Workshop Committee of the Immunology of Diabetes Society.
264		c 2010-10-05
264	1	b Oxford University Press (OUP),c 2011
520		a Autoimmune T cell responses directed against insulin-producing β cells are central to the pathogenesis of type 1 diabetes (T1D). Detection of such responses is therefore critical to provide novel biomarkers for T1D 'immune staging' and to understand the mechanisms underlying the disease. While different T cell assays are being developed for these purposes, it is important to optimize and standardize methods for processing human blood samples for these assays. To this end, we review data relevant to critical parameters in peripheral blood mononuclear cell (PBMC) isolation, (cryo)preservation, distribution and usage for detecting antigen-specific T cell responses. Based on these data, we propose recommendations on processing blood samples for T cell assays and identify gaps in knowledge that need to be addressed. These recommendations may be relevant not only for the analysis of T cell responses in autoimmune disease, but also in cancer and infectious disease, particularly in the context of clinical trials.
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Immunologi inom det medicinska området0 (SwePub)301102 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Immunology in the medical area0 (SwePub)301102 hsv//eng
700	1	a Mannering, S I4 aut
700	1	a Brooks-Worrell, B M4 aut
700	1	a Durinovic-Belló, I4 aut
700	1	a Cilio, Corradou Lund University,Lunds universitet,Diabetes - immunovirologi,Forskargrupper vid Lunds universitet,Diabetes - Immunovirology,Lund University Research Groups4 aut0 (Swepub:lu)endo-cci
700	1	a Wong, F S4 aut
700	1	a Schloot, N C4 aut
710	2	a Diabetes - immunovirologib Forskargrupper vid Lunds universitet4 org
773	0	t Clinical and Experimental Immunologyd : Oxford University Press (OUP)g 163, s. 33-49q 163<33-49x 0009-9104x 1365-2249
856	4	u http://www.ncbi.nlm.nih.gov/pubmed/20939860?dopt=Abstracty FULLTEXT
856	4	u http://dx.doi.org/10.1111/j.1365-2249.2010.04272.xy FULLTEXT
856	4	u https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010910
856	4 8	u https://lup.lub.lu.se/record/1711254
856	4 8	u https://doi.org/10.1111/j.1365-2249.2010.04272.x

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Av författaren/redakt...: Mallone, R; Mannering, S I; Brooks-Worrell, ...; Durinovic-Belló, ...; Cilio, Corrado; Wong, F S; visa fler...; Schloot, N C; visa färre...

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